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Gram staining refers to separating bacterial species into two groups, gram-positive and gram-negative, which defines the physical and chemical properties. Skipping a step in this process could result in a mixed group of the bacterial species.

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10y ago
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13y ago

Heat fixing causes the bacteria in question to affix to the slide. If you skipped this step, the bacterial cells might shift on the slide when you rinse off the different agents, which would render your stain useless since all of the bacteria would be down the drain.

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11y ago

Heat fixing causes the bacteria in question to affix to the slide. If you skipped this step, the bacterial cells might shift on the slide when you rinse off the different agents, which would render your stain useless since all of the bacteria would be down the drain.

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Q: What would happen if the step involving Gram's iodine was inadvertently left out of the Gram staining procedure?
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Related questions

What would happen if no fixing were done?

If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.


What would happen if no heat fixing were done?

If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.


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