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Why does gram plus hold onto crystal violet and gram - become decolorized?
Gram positive bacteria have a thicker cell wall.
Why do gram positive bacteria do not become decolorized?
the thick peptidoglycan with smaller pores enables gram positive cells to retain the primary dye
Why does the crystal violent stain wash out of gram bacteria when they are decolorized in ethanol?
gram negative have a very thin cell wall with an extra membrane layer (lipopolysac charide) out side.
Compare gram-positive bacteria with gram-negative bacteria?
The peptidoglycan layer of the cell wall is thicker for a gram-positive bacterium.
Morphology of the gram-positive organism?
The gram positive cell wall contains a thicker layer of peptydoglacans than that of gram negative. the gram negative bacteria doesn't have a LPS(Lipopolysaccharides) covering outside the cell wall like the gram negatives.their is a periplasmal space between peptidoglycan cell wall and the cell membrane. During Gram's staining the CVI complex doesnt leave the gram positive bacteria because of the thick layer of peptydoglycanes.
Why does gram plus hold onto crystal violet and gram - become decolorized?
Gram positive bacteria have a thicker cell wall.
Why do gram positive bacteria do not become decolorized?
the thick peptidoglycan with smaller pores enables gram positive cells to retain the primary dye
Why do gram positive bacteria retain the primary stain methylene blue while gram negative bacteria are decolorized by rinsing with ethanol?
capsule
Why does the crystal violent stain wash out of gram bacteria when they are decolorized in ethanol?
gram negative have a very thin cell wall with an extra membrane layer (lipopolysac charide) out side.
If your organisms were all blue with pink spots what is the Gram reaction if your organisms were all pink but had some blue spots what is the Gram reaction?
Gram positive cells take up the crystal violet, which is then fixed in the cell with the iodine mordant. This forms a crystal-violet iodine complex which remains in the cell even after decolorizing. It is thought that this happens because the cell walls of gram positive organisms include a thick layer of protein-sugar complexes called peptidoglycans. This layer makes up 60-90% of the gram positive cell wall. Decolorizing the cell causes this thick cell wall to dehydrate and shrink, which closes the pores in the cell wall and prevents the stain from exiting the cell. At the end of the gram staining procedure, gram positive cells will be stained a purplish-blue color. Gram negative cells also take up crystal violet, and the iodine forms a crystal violet-iodine complex in the cells as it did in the gram positive cells. However, the cell walls of gram negative organisms do not retain this complex when decolorized. Peptidoglycans are present in the cell walls of gram negative organisms, but they only comprise 10-20% of the cell wall. Gram negative cells also have an outer layer which gram positive organisms do not have; this layer is made up of lipids, polysaccharides, and proteins. Exposing gram negative cells to the decolorizer dissolves the lipids in the cell walls, which allows the crystal violet-iodine complex to leach out of the cells. This allows the cells to subsequently be stained with safranin. At the end of the gram staining procedure, gram negative cells will be stained a reddish-pink color. Remember:
Is mycolic acid found in gram positive and gram negative cell wall composition?
mycobacterium
Name the different gram positive and gram negative bacteria?
Name of gram positive and gram negative bacteria?
Compare gram-positive bacteria with gram-negative bacteria?
The peptidoglycan layer of the cell wall is thicker for a gram-positive bacterium.
What describes a difference when gram-positive bacteria are compared to gram negative bacteria?
The peptidoglycan layer of the cell wall is thicker for a Gram-positive bacterium.
What describes a difference. When gram-positive bacteria are compared to gram-negative bacteria?
The peptidoglycan layer of the cell wall is thicker for a Gram-positive bacterium.
Morphology of the gram-positive organism?
The gram positive cell wall contains a thicker layer of peptydoglacans than that of gram negative. the gram negative bacteria doesn't have a LPS(Lipopolysaccharides) covering outside the cell wall like the gram negatives.their is a periplasmal space between peptidoglycan cell wall and the cell membrane. During Gram's staining the CVI complex doesnt leave the gram positive bacteria because of the thick layer of peptydoglycanes.
You used acid-alcohol as the decolorizing agent?
If you used acid-alcohol as the decolorizing agent in spore staining the cells of the bacillus are gram plus. If you use acid alcohol it will not decolorize gram plus cell wall as only gram negative are decolorized. It will not get a differentiation in color.
