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5' cap and poly (A) tail
Poly A tail at 3' end

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Q: What helps to stabilize mRNA by inhibiting its degradation?
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When and at what end does the tailing of hnRNA take place?

The tailing of hnRNA, which involves the addition of a poly-A tail to the 3' end of the mRNA molecule, occurs after transcription and before the mRNA is released from the nucleus. This process helps stabilize the mRNA and facilitate its export to the cytoplasm for translation.


What is the purpose of the 5' cap and the 3' poly A tail?

5' cap helps protect mRNA from degradation by hydrolytic enzymes and after mRNA reaches the cytoplasm, the 5' cap functions as part of an "attach here" sign for ribosomes. The poly-A-tail inhibits degradation of RNA and helps ribosomes attach and facilitates export of mRNA from the nucleus.


How cell may vary the amount of enzyme?

Two main ways: synthesis and degradation. Each step in the process leading to synthesis of the enzyme can be regulated - gene expression, mRNA processing and stability, mRNA translation.


What process produces protein from rna?

mRNA - translation tRNA- transcription ending with degradation rRNA- trolletion maximum


What happens to the transcript RNA before it leaves the nucleus?

The 5-prime end is immediately capped off with a modified form of a guanine (G) nucleotide. This 5-prime cap has at least two important functions: protect the mRNA from degradation by hydrolytic enzymes and act as an "attach here" sign for ribosomes. The 3-prime end gains a poly(A) tail consisting of 30 to 200 adenine nucleotides, which also inhibits degradation of the RNA and helps ribosomes attach to it. The poly(A) tail also seems to facilitate the export of mRNA from the nucleus.


What protects mRNA from attack by cellular enzymes?

An eukaryotic mRNA has 2 ends, a 3' (three prime) end and a 5' (five prime) end. They are both protected from degradation. The 3' end is protecting by a long tail of the Adenosine base, this tail is reffered to as the Poly-A tail and is established through the process of polyadenylation. The 5' end has a different method of protection from degradation, it undergoes "capping". Capping involves a Gaunine base paring in a 5' - 5' manner with the exposed 5' end of the mRNA. This basically leaves no exposed 5'. An eukaryotic mRNA has 2 ends, a 3' (three prime) end and a 5' (five prime) end. They are both protected from degradation. The 3' end is protecting by a long tail of the Adenine base, this tail is reffered to as the Poly-A tail and is established through the process of polyadenylation. The 5' end has a different method of protection from degradation, it undergoes "capping". Capping involves a Gaunine base paring in a 5' - 5' manner with the exposed 5' end of the mRNA. This basically leaves no exposed 5'.


How is mRNA stabilized before leaving the nucleus for translation?

Before leaving the nucleus, the mRNA is modified (post-transcriptional modification). It is protected from ribonucleases by adding a 5' cap and a (3') poly A tail. These modifications help to stabilise the mRNA by preventing degradation by nucleases.


What Moves along the mRNA and helps form the peptide strand?

ribosome


What is the step of protein synthesis that occurs in the nucleus?

The step of protein synthesis that occurs in the nucleus is transcription. Transcription is the process of copying the genetic code from DNA into RNA. The RNA molecule that is produced during transcription is called messenger RNA (mRNA). mRNA then travels to the cytoplasm, where it is translated into a protein. In transcription, the DNA double helix opens up at a specific location called the promoter. A protein called RNA polymerase binds to the promoter and begins to move along the DNA strand, unzipping the helix as it goes. RNA polymerase then uses the exposed DNA strand as a template to synthesize a complementary RNA strand. This RNA strand is the mRNA molecule. Once the mRNA molecule is complete, it separates from the DNA and travels to the cytoplasm. In the cytoplasm, the mRNA molecule binds to a ribosome. The ribosome then reads the mRNA molecule and uses the information to assemble a protein molecule. The protein molecule is then released from the ribosome and can go on to perform its function in the cell. Here are the steps of transcription in detail: Initiation: RNA polymerase binds to the promoter region of DNA. Elongation: RNA polymerase moves along the DNA strand, unzipping the helix and synthesizing a complementary RNA strand. Termination: RNA polymerase reaches a termination sequence in the DNA and stops synthesizing RNA. The mRNA molecule that is produced during transcription is a single-stranded molecule that contains the same information as the original DNA strand. However, the mRNA molecule is modified in a few ways. First, the 5' end of the mRNA molecule is capped with a group of nucleotides called a 5' cap. The 5' cap helps to protect the mRNA molecule from degradation and helps it to bind to the ribosome. Second, the 3' end of the mRNA molecule is polyadenylated, which means that it is added to a long chain of adenine nucleotides. The polyadenylation tail helps to stabilize the mRNA molecule and helps it to be transported to the cytoplasm. Transcription is a complex process that is essential for protein synthesis. It is the first step in the process of converting genetic information into a functional protein molecule.


How is mRNA silenced?

mRNA can be silenced through a process called RNA interference (RNAi). In RNAi, small RNA molecules called small interfering RNAs (siRNAs) or microRNAs (miRNAs) bind to the mRNA molecules and prevent them from being translated into proteins. This silencing can occur through mechanisms such as degradation of the mRNA or inhibition of its translation. Additionally, other factors such as RNA-binding proteins and long non-coding RNAs can also contribute to mRNA silencing.


What are mRNP complexes?

It means mRNA protein. They are mainly responsible for the transport of mRNA out of the nuclear pores after transcription. EJC and TAP are some mRNPs. I hope this helps.


What is the importance of regulating gene expression?

It helps you alloe dna replication through mRNA