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By adding a specific reagent.
This is so you can know the base line. The base line is used as the starting point for the mesaurements used for the Rf values. you need to know the distance from the base line to the point of the spot to know the Rf values. see related link below for more info about paper chromatography
yes.
No, but he must run in the "runners box" when he reaches that spot down the first base line, or he may be called out for interference.
The penalty spot is 12 yards from the goal line.
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If you are talking ac, then a spot of frost.If you are talking ac, then a spot of frost.
yes
first base
Tranquility Base
coordinate
A contour line or a spot height.