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You could first verify that you have sulfur reducers in your culture by inoculating the SIM medium and checking for the formation of the black FeS precipitate. Then, you could re-make the SIM medium excluding the ferric ammonium citrate (Fe source) and re-inoculate. There will be no black precipitate formation and you could see growth along the stab line and possibly emanating from it.

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Q: What ingredient or step could be eliminated in the SIM medium were used strictly for testing motility among sulfur reducers?
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