used as a co-factor o th enzime taq polimerase
Taq polymerase, the enzyme used frequently in Polymerase Chain Reaction, is extracted from Thermophilus aquaticus, a thermophilic bacteria.
I is known as Polymerase Chain Reaction (PCR)
Polymerase chain reaction
It is used in Polymerase Chain Reaction (PCR), which embrace temperature of 94°C - the polymerase has to be able to sustain such temperature.
PCR
polymerase chain reaction (PCR)
Unlike Taq DNA polymerase, E.coli DNA polymerase is not heat-stable and will denature during the strand denaturation step of the PCR reaction.
PCR stands for "polymerase chain reaction," which is a molecular biology technique used to amplify and detect specific DNA sequences. It is commonly used in medical diagnostics and research to detect viruses, bacteria, and genetic mutations.
The Polymerase Chain Reaction (PCR) technique employs a heat-stable polymerase in a chain reaction, replicating DNA exponentially.
no e coli DNA pol can not with stand high temperature if so for every denaturation step we must add fresh enzyme
Polymerase Chain Reaction is widely used in many areas to identify DNA and detect infectious organisms or genetic variations, including the viruses that cause AIDS, hepatitis, and tuberculosis, detection of mutations in human genes, and numerous other tasks.
The enzyme DNA polymerase ( Taq polymerase) used in the PCR requires Mg 2+ ions for its functioning.These Ions act as cofactors for the enzyme . Hence the requirement for the use of Mg Cl2 in PCR reactions.