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What else can I help you with?
Which process uses bacteria to copy DNA?
Polymerase chain reaction
Could you use human polymerase for the extension step in the PCR reaction?
Actually the problem with the Human polymerase is the sensitivity to temperature if we talk about PCR. That is the reason why we use Taq DNA polymerase which is thermostable where as use of human polymerase may result in loss of its function due to high temperature.
What chemical is an enzyme that add nucleotides to a new strand of DNA during replication?
DNA Polymerase is the enzyme which adds new nucleotides during replication.
Describe one similarity between PCR and recombinant DNA technology.?
PCR is the abbreviation for polymerase chain reaction. It is similar to recombinant DNA technology in that both have the ability to sequence DNA.
What enzymes are responsible for replication of the DNA molecule?
Helicase is the enzymes that splits the double helix into two separate strands, and DNA Polymerase (as opposed to RNA Polymerase) joins the nucleotides together in the new strands being created.
What is thermostable DNA polymerase?
Thermostable DNA polymerase is an enzyme that can withstand high temperatures, typically used in PCR (polymerase chain reaction) to amplify DNA. The most well-known example is Taq polymerase, which is isolated from the thermophilic bacterium Thermus aquaticus. Its ability to function at high temperatures allows for the repeated cycles of heating and cooling necessary for PCR.
Why is a thermostable polymerase used in PCR?
The thermostable polymerase (or Taq polymerase) is a thermostable DNA polymerase (named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Thomas D. Brock in 1965), is often abbreviated to "Taq Pol" (or simply "Taq"), and is frequently used in polymerase chain reaction (PCR). Taq polymerase is as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR; Therefore it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75-80°C, with a half-life of greater than 2 hours at 92.5°C, 40 minutes at 95°C and 9 minutes at 97.5°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72°C.
Which process uses bacteria to copy DNA?
Polymerase chain reaction
Could you use human polymerase for the extension step in the PCR reaction?
Actually the problem with the Human polymerase is the sensitivity to temperature if we talk about PCR. That is the reason why we use Taq DNA polymerase which is thermostable where as use of human polymerase may result in loss of its function due to high temperature.
Why taq polymerase is thermostable?
Taq Polymerase is an important enzyme component involved in the PCR reaction. Its A DNA polymerase and its role is to elongate the growing strands of DNA during the extension process. Since the Extension process in a PCR works at a temperature which a human DNA polymerase cannot remain active, the Taq polymerase obtained from Thermus aquaticus (living in the hot springs) are used and hence these enzymes are thermo stable.
Why taq polymerase is strored at very low temperature as it is thermostable?
Storing Taq polymerase at a very low temperature (typically -20°C) helps preserve its activity over time. While Taq polymerase is thermostable and can withstand high temperatures during PCR, storing it at low temperatures helps prevent degradation and denaturation of the enzyme, leading to better performance in PCR reactions.
What is difference between recombinant DNA technology and Polymerase chain reaction?
r DNA technology is technology of creating new combination of DNA. While pcr is one of techniques used in r DNA technology for amplification of perticuler DNA fragment
Who discovered taq polymerase enzyme?
Alice Chien, et al. were the first to describe the Taqpolymerase in 1976.Then, Saiki et al. were the first to employ the polymerase in a published study.Chien A, Edgar DB, Trela JM. Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus. J Bacteriol 1976;127:1550-1557.Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988;239:487-491.
What chemical is an enzyme that add nucleotides to a new strand of DNA during replication?
DNA Polymerase is the enzyme which adds new nucleotides during replication.
Describe one similarity between PCR and recombinant DNA technology.?
PCR is the abbreviation for polymerase chain reaction. It is similar to recombinant DNA technology in that both have the ability to sequence DNA.
Is the enzyme that allows replication to occur DNA polymerase or RNA polymerase?
DNA polymerase replicated DNA. RNA polymerase creates mRNA to be used in protein synthesis. RNA polymerase does not replicated DNA.
Is DNA polymerase a protein?
Yes, DNA polymerase is a protein.
