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How often does DNA polymerase match bases incorrectly?
DNA polymerase has an error rate of approximately 1 in every 10^7-10^8 nucleotides incorporated. This means that it matches bases incorrectly about once in every 10 million to 100 million nucleotides during DNA replication.
A source of heat-stable DNA polymerase is?
A DNA polymerase is one of the crucial enzymes when DNA is synthesised. It is also the only enzyme needed when making DNA in the test tube, using a molecular biology technique known as PCR.In this reaction, the other enzymes that nature uses are are replaced by cycles of heating and cooling, up to 95 degrees Celsius. The DNA polymerase consists of protein, so normal DNA polymerase is of course destroyed in the heating step. The first PCR's were performed by opening the tube in each step and adding a tiny amount of fresh enzyme. Using heat-stable DNA polymerases made the technique a lot more practical. The enzymes used were taken från a type of archae (not exactly a bacterium, but almost) that live in hot springs and whose proteins all are very stable even in extreme temperatures. The archaeon is called "Thermus aquaticus", hence the name of the common lab DNA polymerases "Taq polymerase":To sum it up. A heat-stable DNA polymerase is a kind of DNA polymerase found in archaea living in hot springs, and of much use in the molecular biology lab.
Can polymerase catch and correct every reptication error?
Yes. Initially, DNA replication makes 1 mistake in a 100,000. Like spell check, DNA polymerase comes in and removes errors in base pairs and correct them by adding the right ones. After DNA polymerase checks the new strand for errors, the end result is 1 mistake in a billion. If this didn't occur, mutations would surely take place in out body.
Why normal DNA polmerase not use in PCR?
In the PCR, high temperatures are used in order to separate both strands of DNA readily. Normal DNA polymerases would "melt" (denature) under these conditions, whereas Taq DNA Polymerase does not (short from Thermus aquaticus, a bacteria that lives in very hot submarine springs).
What is used to make copies of DNA?
Gel electrophoresis can be used to separate various pieces of DNA by their lengths. When the location of target strands of DNA need to be located, specific restriction enzymes function to sever the particular DNA strand, and then take the desired, different strands of DNA (severed by the same restriction enzyme) and adds it to the original specimen of DNA. I know this works on plasmids (circular pieces of DNA found in bacteria). Very, very interesting stuff.
Role of Taq polymerase in PCR?
taq polymerase is special because it is very stable at high temperatures and will not denature even at the 90 degree step of pcr. taq polymerase is so heat stable because it was extracted from the bacterium thermus aquaticus, which is found in hot springs and geezers
Application of Polymerase Chain Reaction to Adenovirus and Herpavirus and Pox-virus?
it is very good
Compare the polymerase chain reaction and DNA replication?
PCR need thermostable enzyme like taq DNA polymearse, while in replication using highly proofreading enzyme DNA polymerase. taq enzyme work in very high temprature while in replication our body temprature
What is the explanation for the Role of RNA polymerase?
Basically, RNA polymerase's role is very similar to that of DNA polymerase. RNA polymerase is an enzyme that is used during transcription in the nucleus. Similar to DNA polymerase, RNA polymerase codes for the complementary nucleotides to a DNA strand. Instead of thymine though, uracil codes with adenine. This coded mRNA strand then travels from the nucleus to the ribsome where translation occurs - the result is protein made from an amino acid chain. To answer your main question - RNA polyermase adds the complementary nucleotides to the DNA strand using uracil instead of thymine. hope that helps :)
How does Polymerase Chain Reaction work?
Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a mixture of DNA molecules.
What is the temperature in the summer for Chad?
The temperature is very hot
What is the temperature range in Ontario?
The average temperature is
What is the Neptune's surface temperature?
The surface temperature on Neptune is very very cold. it is - 391 F.
What is the temperature of Pluto's core?
very, very cold
At whish temperature does chocolate melt?
it melts at a very hot temperature
A gas that burns at a very high temperature?
which gas burns at a very high temperature
Someone who studies matter at a very low temperature?
Someone who studies matter at a very low temperature is called a cryogenicist.
