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What is Nitroprusside?

Updated: 10/24/2022
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6y ago

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They are two tests that a laboratory would perform on urine to screen for metabolic diseases. The first is to detect ketoaciduria and the second to detect cystinuria/homocystinuria.

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Kayleigh Waelchi

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Q: What is Nitroprusside?
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Why do you prepare lassaigne extract?

lassaigne extractA small piece of sodium is heated gently in an ignition tube till the sodium melts. About 50 to 60 mg of the organic compound is added to this and the tube heated strongly for 2-3 minutes to fuse the material inside it. After cooling, the tube is carefully broken in a china dish containing about 20 to 30 mL of distilled water. The fused material along with the pieces of ignition tube is crushed with the help of a glass rod and the contents of the china dish are boiled for a few minutes. The sodium salts formed in the above reactions (i.e. NaCN, Na2S, NaX or NaSCN) dissolve. Excess of sodium reacts with water to give sodium hydroxide. This alkaline solution is called Lassaigne's extract or sodium extract. The solution is then filtered to remove the insoluble materials and the filtrate is used for making the tests for nitrogen, sulphur and halogens.ReactionsAn organic compound containing C, H, N, S and halogens when fused with sodium metal gives the following reactions.(NaSCN) is formed during fusion, which in the presence of excess sodium forms sodium cyanide and sodium sulphide.Detection of nitrogenA small quantity of the sodium extract is taken in a test tube. It is made alkaline by adding 2-3 drops of sodium hydroxide (NaOH) solution. 1 mL of freshly prepared solution of ferrous sulphate is added to this solution. The mixture of the two solutions is boiled and then acidified with dilute sulphuric acid. The appearance of prussian blue or green colouration of the precipitate confirms the presence of nitrogen in the given organic compound. The carbon and nitrogen present in the organic compound on fusion with sodium metal give sodium cyanide (NaCN) soluble in water. So, the sodium extract contains sodium cyanide which, on reaction with ferrous sulphate, gives sodium ferrocyanide. Some of the ferrous salt is oxidised to the ferric salt on heating and this reacts with sodium ferrocyanide to form ferric ferrocyanide.Note: When nitrogen and sulphur both are present in any organic compound, sodium thiocyanate is formed during fusion. When extracted with water sodium thiocyanate goes into the sodium extract and gives 'blood red coloration' with ferric ions due to the formation of ferric thiocyanate.Detection of sulphurThe presence of sulphur in any organic compound is detected by using sodium extract as follows:Lead acetate testA small portion of sodium extract is acidified with acetic acid and lead acetate solution is added to it. A black precipitate of lead sulphide indicates the presence of sulphur.Sodium nitroprusside testTo a small quantity of sodium extract taken in a test tube, 2 to 3 drops of sodium nitroprusside are added to the solution. A violet color indicates the presence of sulphur. This color fades away slowly on standing.by: zoren roicres apostol. copy and pastesource:http://www.tutorvista.com.


Related questions

What is the positive result of nitroprusside test?

RED


Color of acetone after adding sodium nitroprusside?

Red


What amino acids are found in nitroprusside test?

cystine,cysteine,methionin


How does nitroprusside affect cardiac output?

Sodium nitroprusside is used in emergency to reduce the very high blood pressure. The drug dilates the arterial as well as venous system. So you get tachycardia and cardiac out put increases probably.


What happens when potassium sulfide reacts with sodium nitroprusside?

solution becomes violet in colour


What is the formula of the colored precipitate obtained in the nitro prusside test?

Sodium nitroprusside is used for detection of sulphur in organic compounds. If sulphur is present in the organic compound, sodium fusion will convert it into sodium sulphide. Sulphide ions are readily identified using sodium nitroprusside. Na + S = Na2S Na2s + Na2[Fe(CN)5NO] = Na4[Fe(CN)5NOS] Sodium nitroprusside violet colour Submitted by Dr Arun Trikha on July 22nd 2012 .


What is the significance of sodium-nitroprusside test?

This test is performed to test the presence of cysteine in the test solution (protein solution)


What is the goal of combined dopamine and nitroprusside therapy?

Dopamine vasoconstricts, so it increases the blood pressure in cardiogenic shock. Nitroprusside is a vasodilator, it decreases preload (left ventricular stretch) and afterload (resistance). They are used in combination in cardiogenic shock to achieve a good hemodynamic effect or "good balance" or adequate blood flow.


What effect does Sodium Nitroprusside have on afterload and preload?

It decreases preload and afterload as a result of the dilation in the venous and arterial vasculature from the nitric oxide.


What is phenylhydrazine-nitroprusside test?

They are two tests that a laboratory would perform on urine to screen for metabolic diseases. The first is to detect ketoaciduria and the second to detect cystinuria/homocystinuria.


How can medication cause hypothyroidism?

Nitroprusside, lithium, or iodides can induce hypothyroidism. Because patients who use these medications are closely monitored by their doctors, this side effect is very rare.


What is the Chemical reaction of acetone in 0.5 percent sodium nitroprusside?

CH3COCH3 + Na2[Fe(CN)5NO]~Na2[Fe(CN)5HO-NCHCOCH3](WINE RED PPT)