Reverse transcription polymerase chain reaction (RTPCR) relies on chemical analysis of.(RNA) to: evaluate the effectiveness of cancer therapies.identify mutations.reveal cancer that recurs after treatment has been completed
The common cold is only caused by viruses. Other viruses can also cause similar symptoms (for example, the flu virus), however. In addition, there can be upper respiratory bacterial infections (like tonsillitis or strep throat making a sore throat, sinus infections making a runny nose, bronchitis making a cough, etc.) that all may seem like a cold. The typical difference between viral infections and bacterial infections is that bacterial infections more often produce fever and more often cause high fever over 102 degrees Fahrenheit, but sometimes only a doctor can tell the difference (and sometimes, even then, it takes laboratory testing to prove one microbe versus the other).
RNAse destroys the RNA and hence RNAse contamination is a problem in RNA extraction as it breaks down RNA. RNAse enzyme is removed by using RNAse inhibitor or precautions like wearing of gloves, autoclaving tips , using RNAse free water/DEPC treated water is done while performing RTPCR