Considering this question doesn't make sense, there's not any way to answer it. :D bai.
When a large part of DNA is inserted into a gene, it is referred to as a "gene insertion" or "gene modification." This process can lead to the addition of new genetic information, potentially altering the function of the gene and affecting the organism's traits. Such modifications can be achieved through techniques like gene editing or recombinant DNA technology.
Genes carry instructions for assembling proteins, which are essential for various biological processes in cells. These instructions are encoded in the DNA sequence of the gene and are transcribed and translated into proteins.
For rejoining DNA fragments, you can use DNA ligase enzyme, which catalyzes the formation of phosphodiester bonds between adjacent DNA fragments. This process is commonly used in molecular biology techniques like PCR and gene cloning.
Proteins determine how a gene is expressed. Proteins are composed of amino acids that are synthesized (put together) by RNA, and RNA is made from DNA. DNA is what you inherit from your parents--very basically, your genes are sections of DNA that code for certain proteins (that are composed of amino acids).
Large quantities of protein can be produced by expressing the gene of interest in a bacterial colony such as E. coli. This is typically achieved by cloning the gene into a plasmid, transforming the plasmid into the bacterial cells, and inducing protein expression. The bacterial colony can then be grown in a culture medium optimized for protein production to maximize yields.
it would be 1st chromosome. then gene. then DNA
Genes carry instructions for assembling proteins, which are essential for various biological processes in cells. These instructions are encoded in the DNA sequence of the gene and are transcribed and translated into proteins.
No. DNA is not put together randomly. The sequence of DNA is specific to the genes that control the cell's activities and heredity. Each gene has its unique sequence.
A recombinant sequence of DNA is a sequence of DNA that comes from more than one source. Examples of recombinant DNA are plasmids that are put into bacteria. The plasmid comes from the bacteria (or a bacteria at least) but a target gene has been added (say the lac operon gene that allows bacteria to thrive on lactose), this plasmid is now a recombinant DNA sequence.
first you have to take the gene of interest (the gene that you want to put into the bacteria) and get it cut with restriction enzymes. then mix it with the plasmids from the bacteria. then you put the mixture, which is now called a recombinant DNA into the bacteria and incubate it so it can grow.
For rejoining DNA fragments, you can use DNA ligase enzyme, which catalyzes the formation of phosphodiester bonds between adjacent DNA fragments. This process is commonly used in molecular biology techniques like PCR and gene cloning.
The 4 steps of genetic engineering are DNA cleavage and isolation, production of recombinant DNA cloning, and screening. DNA cleavage and isolation uses restriction endonucleases to break up DNA strands. The production of recombinant DNA inserts DNA fragments into plasmids or similar vectors also digested by same restriction enzymes.
1. A vector such as plasmid is needed along with a host cell. Restriction enzymes and DNA ligase are enzymes that are used to introduce foreign DNA into a vector.
Yes, there are two similar techniques in which i am aware of.AdenovirusesFirstly adenoviruses are made harmless by interefering with a gene involved in replication. A healthy from of the CFTR gene is extracted and cut with restriction endonucleases, the same enzyme is used to cut a bacterial plasmid. The gene and plasmid are mixed together along with DNA ligase to anneal the phosphosugar framework of the DNA fragment and bacterial plasmid. The plasmid is then mixed with epithelial cells. The plasmid is then isolated and purified and places into adenoviruses. These are then placed onto the nostrils of individuals with cystic fibrosis. The viruses find their way to epithelial cells in the airways and injected their DNA. The DNA contains the functional CFTR gene, the cells can then produce fucntional CFTR proteins.LiposomesA healthy gene is extracted from a human. This gene is then inserted into a bacterial plasmid, in a similar manner as discussed above. The bacterial plasmids are then inserted into bacteria. These are allowed to grow and divide, producing large quantities of the plasmid, with the required gene. These plasmids are then extracted and coated in a lipid soluble substance. They are then put into nasal sprays and sprayed onto the nostrils of effected individuals.
The following earlier provided answer, I believe, is wrong:"A gene is not a single structure or thing. It is made up of the micro-structures that determine a trait. These micro-structures are primarily chromosomes. Chromosomes are pieces of DNA. A single strand of DNA stretched out would be about 3 feet long. A common analogy is that DNA is like the strands of a necklace and the chromosomes are the beads. There are two strands of DNA that intertwine forming a pattern called the "Double Helix"basically, a gene is part of a chromosome. which all falls underneath DNA"To my understanding, a chromosome consists of a single DNA string (up to 2 meters long if unfolded) that is tightly curled up around many protein molecules (histone) and around itself (much like old telephone wires after years of use). The resulting bundle is called a chromosome.A Gene is a particular section of the chromosome's DNA string. There are many such sections on the DNA string - thus it contains (also calls 'codes') many genes - in humans between 500 and 4500 depending on what chromosome. One gene contains information for producing one particular protein. It is the sequence of bases (T, C, G or A) in the DNA gene section that defines which protein.The beginning and end of a gene section is marked by a start and a stop sequence ( also called codons) of a gene. In DNA the start can typically be the base sequence ATG and the stop sequence typically TAA, TGA or TAG.Humans have 23 pairs of chromosomes. Each chromosome has a different DNA string. Thus some times you see people wrinting that a chromosome is only a part of a person's DNA. By that, they mean all the DNA in the body - whcih of course is true, since you have 23 different pairs of chromosomes. But that should not be misunderstood as "a chromosome is part of a DNA string" as frequently seen on the net.Genes are to be found on Chromosomes.And Chromosomes are singular pieces of DNA, which contain many genes, regulatory elements and other nucleotide sequences.
I had the same problem but i found out that a gene is a small segment of a DNA strand. I'm sorry i don't know how long the gene segment is but DNA is made up of many genes segments put together.
Proteins determine how a gene is expressed. Proteins are composed of amino acids that are synthesized (put together) by RNA, and RNA is made from DNA. DNA is what you inherit from your parents--very basically, your genes are sections of DNA that code for certain proteins (that are composed of amino acids).