Acetocarmine stains chromosomes and sterile pollen must lack them.
it is a genetic stain by which chromosomes can be distinctly seen
In pollen context, acetocarmine is used as a weak stainer for cytoplasm, unlike in chromosome studies where it stains nucleic acids.
Chromosomes are easiest to see when they are condensed(coiled and supercoiled, so that they become short and fat).They are at their most condensed from late prophase through to early telophase, in either mitosis or meiosis.Their visibility also depends on suitable microscopic technique, including the use of an appropriate stain, such as acetocarmine.
Surfectants work in stain removers to remove the stain and not damage what the stain is on. It oxidizes the stain and lifts it out of whatever its in,
Acetocarmine stains chromosomes and sterile pollen must lack them.
Cochineal (Dactylopius coccus)
it is a genetic stain by which chromosomes can be distinctly seen
Acetocarmine is a DNA specific stain like feulgen stain, so the super coiled chromosomes during different stages of mitosis present in the Onion root tip cells can be visualized perfectly by treating with this stain.
we use acetocarmine to stain chtomosomes. stained chromosomes distinguish from the other organels and materials, it gives the chromosomes a red color. it enables that, during the cell cyle we can observe the nucleus, duplication of DNA, and movement.
In pollen context, acetocarmine is used as a weak stainer for cytoplasm, unlike in chromosome studies where it stains nucleic acids.
take the root tip (about 1 cm )and put it in dil. HCL solution for ten min. take the tip out and put in acetocarmine stain for 1min. take out the root put it in water bto remove extra stain .
Acetocarmine is acetic acid as solvent with dissolved carmine
Chromosomes are easiest to see when they are condensed(coiled and supercoiled, so that they become short and fat).They are at their most condensed from late prophase through to early telophase, in either mitosis or meiosis.Their visibility also depends on suitable microscopic technique, including the use of an appropriate stain, such as acetocarmine.
The difference between Wright Stain and Giemsa Stain is the intensity of the stain. The Giemsa Stain provides a better stain intensity than the Giemsa stain.
yes you can and the stain can change colors if there is a juice stain for example thats red and a grass stain the stain color could change
a huge stain. a jumbo stain. a large stain.