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culture is stabbed deeply into the agar using an inoculating needle....
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Why important to use Agar Deep Stab medium soon after preparation?
Over time, free oxygen will diffuse into the agar.
What is the purpose of motility agar?
Motility Test Agar is used for the differentiation of microorganisms on the basis of motility.Many bacteria have flagella that make them capable of swimming through water-based environments. Motility can be determined by observing cells in a wet mount. However this determination can be difficult because bacteria are small enough that they are bounced around by water molecules. This random movement, called Brownian motion, can be confused with self-propelled motility. Another way to determine motility is with a Motility Agar stab. Motility Agar is soft agar in a test tube (without a slanted surface). Cells are stab-inoculated into the agar (the top surface is not inoculated). Non-motile bacteria will only grow where they were inoculated. Motile bacteria will grow along the stab and will also swim out away from the stabbed area. Thus, a negative result is indicated by growth in a distinct zone directly along the stab. A positive result is indicated by diffuse (cloudy growth), especially at the top and bottom of the stab. Results {| ! scope="col" | + - Positive (cloudy, diffuse growth)! scope="row" | - = Negative (well-defined growth along the stab).! scope="row" | V = Variable! scope="row" |! scope="row" || CAUTION: This test only works with organisms that can grow anaerobically (e.g., facultative anaerobes). Obligately aerobic organisms will grow on the top of the agar but will not grow in the stab.|}
What is the purpose of stabbing into the blood agar?
Streak stab is most commonly used when inoculating blood agar with hemolytic bacteria. The stabs expose the bacteria to a greater surface area of blood cells and will enhance the hemolysis effect.
What is an agar-agar?
An agaric is any of various types of fungi of the family Agaricaceae.
What is the material used as a base for bacterial culture media?
The substance is Agar or Agar-Agar
Why important to use Agar Deep Stab medium soon after preparation?
Over time, free oxygen will diffuse into the agar.
Why did you stab the peptone iron agar instead of inoculating only the surface?
Because the peptone iron agar is used to detect ANAEROBIC bacteria. If you stab it deep into the agar you allow the bacteria to grow in the absence of oxygen. If you only inoculated the surface the bacteria wouldn't grow.
Why is the streak-stab technique on blood agar preferred over incubating the plates anaerobically?
Because Pikachu is the best.
What is the purpose of motility agar?
Motility Test Agar is used for the differentiation of microorganisms on the basis of motility.Many bacteria have flagella that make them capable of swimming through water-based environments. Motility can be determined by observing cells in a wet mount. However this determination can be difficult because bacteria are small enough that they are bounced around by water molecules. This random movement, called Brownian motion, can be confused with self-propelled motility. Another way to determine motility is with a Motility Agar stab. Motility Agar is soft agar in a test tube (without a slanted surface). Cells are stab-inoculated into the agar (the top surface is not inoculated). Non-motile bacteria will only grow where they were inoculated. Motile bacteria will grow along the stab and will also swim out away from the stabbed area. Thus, a negative result is indicated by growth in a distinct zone directly along the stab. A positive result is indicated by diffuse (cloudy growth), especially at the top and bottom of the stab. Results {| ! scope="col" | + - Positive (cloudy, diffuse growth)! scope="row" | - = Negative (well-defined growth along the stab).! scope="row" | V = Variable! scope="row" |! scope="row" || CAUTION: This test only works with organisms that can grow anaerobically (e.g., facultative anaerobes). Obligately aerobic organisms will grow on the top of the agar but will not grow in the stab.|}
What is the purpose of stabbing into the blood agar?
Streak stab is most commonly used when inoculating blood agar with hemolytic bacteria. The stabs expose the bacteria to a greater surface area of blood cells and will enhance the hemolysis effect.
When inoculating agar deep stabs why do you have to insert and remove the needle along the same stab line?
To minimize the introduction of unwanted oxygen into the medium.
Agar agar grain found in Germany?
Agar, or agar-agar, is not a grain, but rather an extract of seaweed. Agar translates to German as Agar-Agar Try whole- or health-food stores
Is there a microorganism that degrades Agar agar?
Yes! Paenibacillus spp. can degrade agar agar
What jelly is made from seaweed and used in glue making?
Agar-agar
Explain the function of the ferrous ammonium sulfate in SIM agar?
Its an indicator by combining with the gas forming an insoluble black ferrous sulfide precipitate that is seen along the line of the stab inoculation and is indicative of H2S production
What is the difference between stab and streaking surface methods of inoculation?
In stabbing, it means pushing the needle to the bottom part of the tube while streaking, it is having a line in a zig-zag manner over the surface of the agar.
What does the naming agar as agar agar denote?
an extract of seaweed
