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What is an example of a gel?
example of gel is agarose gel,
What is a difference between 2 percent and 3 percent agarose gel?
the thickness of the gel varies as you change the conc. of agarose from 2 to 3 percent. the conc. of loading of samples that gel can handle varies.
What are the objectives of agarose?
The agarose gel acts as a matrix that slows down the dna segments as they move to the opposite charged end of the gel. A larger segment will have a tougher time moving through the gel, while a smaller segment will move faster because it is easier to move it through the gel.
Why you have the range of concentration of agarose in gel electrophoresis?
increasing the agarose concentration will enable the separation of smaller fragments of DNA. the structure of the gel (agarose) consists of crosslinks, therefore the higher the concentration of agarose the more crosslinks there will be and smaller size "holes" for the DNA to travel through (also the other way around, with less concentrated agarose)
What is an agarose gel and what is it used for?
Agarose gel electrophoresis is the easiest and most common method used in biochemistry and molecular biology in separating DNA or RNA molecules according to their size.
What is an example of a gel?
example of gel is agarose gel,
Preparation of 1 percent agarose gel or How to prepare 1 percent agarose gel?
Check the answer for How do you make an electrophoresis gel?
What is role of agarose of electrophoresis?
agarose helps in the separation of DNA bands by controlling the pore size of agarose gel
Is agarose gel electrophoresis suitable for only gram negative plasmid DNA?
Agarose gel electrophoresis is suitable for ALL DNA.
What holds agarose gel together?
Stuff
What is agarose and what is the TM value of agarose?
Agarose is a linear polysaccharide used for gel mediums. Tm (melting temp) is about 85 C.
What does the accuracy of the fragment sizes tell you about the resolving ability of agarose-gel electrophoresis?
The larger fragements will not be very accurate because they cannot resolve in high consentrations of the agarose in the gel. The percent of agarose in the gel affects the ability to resolve larger fragements of DNA
What is a difference between 2 percent and 3 percent agarose gel?
the thickness of the gel varies as you change the conc. of agarose from 2 to 3 percent. the conc. of loading of samples that gel can handle varies.
What are the objectives of agarose?
The agarose gel acts as a matrix that slows down the dna segments as they move to the opposite charged end of the gel. A larger segment will have a tougher time moving through the gel, while a smaller segment will move faster because it is easier to move it through the gel.
Why you have the range of concentration of agarose in gel electrophoresis?
increasing the agarose concentration will enable the separation of smaller fragments of DNA. the structure of the gel (agarose) consists of crosslinks, therefore the higher the concentration of agarose the more crosslinks there will be and smaller size "holes" for the DNA to travel through (also the other way around, with less concentrated agarose)
What is an agarose gel and what is it used for?
Agarose gel electrophoresis is the easiest and most common method used in biochemistry and molecular biology in separating DNA or RNA molecules according to their size.
Why agarose gel electrophoresis is not used for protein analysis?
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