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What else can I help you with?
Why light band come in agarose gel for amplified PCR product?
you mean faint band? you must have to optimize it. There might be several reasons, you have to play around with the PCR program, concentration of dNTPs, co factors such as Mg or Mn, etc.
What is the function of a silica gel in a circuit?
Property of Silica gel is to absorb the moisture. Colour of dry silica gel is blue and it turns into pink when it is saturated with moisture. When the colour of silica gel turns out pink, the wet silica gel is removed and the fresh silica gel is filled. The wet silica gel can be heated and made dry for reuse.
Difference between vertical and horizontal gel electrophoresis?
Horizantal gel electrophoresis is generally used for RNA/DNA based studies, while vertical gel electrophoresis is used for protein based studies.
What is the role of formaldehyde and formamide in RNA gel electrophoresis?
Denatures the RNA
How can scientists determine a genetic sequence using a gel?
DNA polymerase with a mix of nucleotides and labelled dideoxynucleotides
What is a difference between 2 percent and 3 percent agarose gel?
The main difference between a 2% and a 3% agarose gel is the concentration of agarose in the gel. A 3% agarose gel will have a higher agarose concentration, resulting in a higher resolving power for separating larger DNA fragments compared to a 2% agarose gel. However, a higher percentage agarose gel may also have a tighter mesh size, making it harder for larger DNA fragments to migrate through the gel.
How do you get agarose gel to solidify when it doesn't solidify after 10 min in the refrigerator?
Try increasing the concentration of agarose in your gel mixture or extending the cooling time in the refrigerator. You can also check if the agarose powder is expired or if there was an error in the preparation process. If the issue persists, consider using a different brand or batch of agarose.
What are common troubleshooting steps for resolving issues with agarose gel electrophoresis?
Common troubleshooting steps for resolving issues with agarose gel electrophoresis include checking the quality of the agarose gel, ensuring proper buffer preparation and pH, verifying correct voltage and running time, confirming proper loading of samples, and troubleshooting equipment issues such as power supply or gel box problems.
What is an example of a gel?
example of gel is agarose gel,
Why is agarose the preferred substance for creating the gel matrix in gel electrophoresis?
Agarose is preferred for creating the gel matrix in gel electrophoresis because it forms a stable and uniform matrix that allows DNA molecules to move through it effectively based on their size. Agarose gels have a high resolution, meaning they can separate DNA fragments of different sizes accurately. Additionally, agarose is non-toxic, easy to prepare, and can be easily disposed of after use.
What is a common name for gel electrophoresis?
Agarose gel electrophoresis.
Use of agrose in DNA isolation?
Agarose gel is used to separate DNA fragments based on size during electrophoresis. Agarose forms a matrix through which DNA molecules move under an electric field. This helps in visualizing and analyzing DNA samples by separating them according to their size.
Is agarose gel electrophoresis suitable for only gram negative plasmid DNA?
Agarose gel electrophoresis is suitable for ALL DNA.
What are the differences between agarose gel electrophoresis and SDS-PAGE techniques for separating and analyzing biomolecules?
Agarose gel electrophoresis separates biomolecules based on size and charge, while SDS-PAGE separates based on size and mass. Agarose gel is used for larger molecules like DNA and RNA, while SDS-PAGE is used for proteins. Agarose gel uses a gel made from agarose, while SDS-PAGE uses a gel made from polyacrylamide.
What does the accuracy of the fragment sizes tell you about the resolving ability of agarose-gel electrophoresis?
The larger fragements will not be very accurate because they cannot resolve in high consentrations of the agarose in the gel. The percent of agarose in the gel affects the ability to resolve larger fragements of DNA
What holds agarose gel together?
Stuff
What is agarose and what is the TM value of agarose?
Agarose is a linear polysaccharide used for gel mediums. Tm (melting temp) is about 85 C.
