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Durning agarose electrophoresis of plasmid DNA why a smiling band does comes?
A smiling band in agarose gel electrophoresis of plasmid DNA typically occurs when the DNA sample is overloaded on the gel. This results in the DNA migrating in a circular or smile-like pattern rather than a straight band. It's important to load the appropriate amount of DNA to prevent smiling bands and ensure accurate analysis.
What is the visual indication of a supercoiled plasmid on a gel electrophoresis image?
On a gel electrophoresis image, a supercoiled plasmid appears as a tight, condensed band that migrates faster than other forms of the plasmid, such as linear or relaxed circular forms.
What will be the positions of genomic DNA plasmid DNA and RNA in the agarose gel electrophoresis?
RNA band will be near the wells being single stranded,Genomic DNA will be at the centre of the gel being linear double stranded and plasmid DNA being circular moves faster and will be the brightest hence will be near the base..
How can i know if my bacteria contain plasmid or not?
You can determine if your bacteria contain a plasmid by performing a plasmid extraction followed by gel electrophoresis to visualize the presence of plasmid DNA. Other methods include PCR amplification of plasmid-specific sequences or using molecular biology techniques like restriction enzyme digestion to confirm the presence of a plasmid.
You have propagated a vector in a non expression host and isolated the plasmid How can you confirm whether the plasmid that you got is exactly the vector which you propagated?
Check the size in agarose gel, extract it from the gel then purify it and grow it on selective plate.
Can the size of a supercoiled plasmid DNA be determined by using standard DNA fragments electrophoresed in parallel?
Yes, the size of a supercoiled plasmid DNA can be estimated by running standard DNA fragments with known sizes in parallel on an agarose gel. By comparing the migration distance of the supercoiled plasmid with the standard DNA fragments, an approximate size can be determined.
Does an f strain donates a plasmid during conjugation?
No, in conjugation, the F strain serves as the donor of the plasmid (F factor) to the recipient cell. This transfer of genetic material allows the recipient cell to acquire the ability to carry out conjugation itself.
Genetic engineers found a suitable vector to transport a gene from one plant to another in?
the Ti plasmid
How do you find suitable plasmid?
if you are looking for a repository grade plasmid you should search the link below but beware of what you buy through some of the more shady vendors here: importer.ec21.com/buy_leads/plasmid.html
What is plasmid profiling?
Plasmid profiling is a molecular biology technique used to analyze and characterize plasmids—small, circular DNA molecules found in bacteria. This method involves extracting plasmid DNA from bacterial cells and then using techniques such as gel electrophoresis or restriction enzyme digestion to visualize and differentiate the plasmids based on their size and restriction patterns. Plasmid profiling is useful in various applications, including understanding genetic diversity, tracking the spread of antibiotic resistance, and studying bacterial evolution.
The plasmid sample is supposed to contain only the DNA fragmentWhy are there more than one band?
Probably the plasmid is partially uncutted and it is present in his coiled and supercoiled forms. you can add another control as the undigested construct. In addition you can add much more enzyme, change batch of EcoRI and digest overnight with BSA to exclude star activity.
Which is the plasmid that increases resistance to antibiotics?
R-plasmid
