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How to do the conversion between OD and nmol of RNA oligo?
There is a precise formula for the conversion between OD and nmol of agomir/ antagomir, In general, there is a simple calculation as follows. Agomir: 1 OD duplex=2.5 nmol = 33µg Antagomir: 1 OD duplex=5 nmol = 33µg Creative Biogene
What type of modification was made for agomir/ antagomir?
The Chemically-modified Construction of Antagomir and Agomir: Antogimir: 3' terminal was modified by cholesterol, there are two thiols modification in the 5' terminal, four thiols modification in the 3' terminal, and the whole strand is modified by 2'-OMe. Agomir: in the antisense strand, 3' terminal was modified by cholesterol, there are two thiols modification in the 5' terminal, four thiols modification in the 3'terminal, and the whole strand is modified by OMe. Creative Biogene
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