There is a precise formula for the conversion between OD and nmol of agomir/ antagomir, In general, there is a simple calculation as follows.
Agomir: 1 OD duplex=2.5 nmol = 33µg
Antagomir: 1 OD duplex=5 nmol = 33µg
Creative Biogene
Transcription is the process of converting DNA into RNA, while translation is the process of converting RNA into proteins. To differentiate between the two, remember that transcription involves copying genetic information from DNA to RNA, while translation involves reading the RNA code to build proteins.
Translation is the conversion of an RNA message into a sequence of amino acids, forming a protein.
RNA contains nucleotides, which are the building blocks of RNA molecules. Nucleotides are connected in a specific sequence to form the RNA molecule.
How to isolate mRNA Minou Nowrousian Background: mRNAs (messenger RNAs) comprise only a small percentage of all RNA species in a eukaryotic cell, in Neurospora usually ~ 1-6 % (Lucas et al., 1977; Sturani et al., 1979). For some applications like preparation of a cDNA library, target preparation for microarray hybridizations or Northern blot analysis of weakly expressed genes, enriched mRNA preparations are preferable to total RNA. Enrichment of eukaryotic mRNAs derived from nuclear encoded genes is done by virtue of their poly(A) tail which in most cases is 30-200 nt long. mRNA or poly(A)-RNA preparation consists of three steps: (1) hybridization of poly(A)-containing RNAs to oligo-dT molecules connected to a carrier, (2) washing off nucleic acids which do not bind to oligo-dT
RNA polymerase catalyzes the linkage between nucleotides to form mRNA during the process of transcription. Reverse transcriptase is an enzyme that synthesizes DNA from an RNA template, tRNA is involved in protein synthesis, and RNA ligase is responsible for joining RNA molecules together.
the difference is that DNA is a double helix and RNA is a single chain
Hydrogen bonds are indeed present in RNA, particularly between complementary bases in the double-stranded regions of RNA molecules, such as between adenine and uracil (or thymine) and between guanine and cytosine. These hydrogen bonds are essential for maintaining the structure and stability of RNA molecules.
There are three main differences between RNA and DNA: The sugar in RNA is ribose instead of deoxyribose, RNA is generally single-stranded, and RNA contains uracil in place of thymine.
Bacteria has both DNA and RNA where as Virus has either DNA or RNA
Comparing DNA and RNA, some key differences include: DNA is double-stranded, while RNA is single-stranded; DNA contains deoxyribose sugar, RNA contains ribose sugar; DNA has thymine base, RNA has uracil base; DNA is found in the nucleus, RNA is found in the cytoplasm; DNA is stable, RNA is less stable; DNA is the genetic material, RNA is involved in protein synthesis. These are just a few of the many distinctions between DNA and RNA.
RNA is a polymer of ribonucleic acids. Amino acids are the monomers of proteins.
The three main structural differences between DNA and RNA are: DNA is double-stranded, while RNA is single-stranded. DNA contains the sugar deoxyribose, while RNA contains the sugar ribose. DNA contains the base thymine, while RNA contains the base uracil instead.