answersLogoWhite

0

There is a precise formula for the conversion between OD and nmol of agomir/ antagomir, In general, there is a simple calculation as follows.

Agomir: 1 OD duplex=2.5 nmol = 33µg

Antagomir: 1 OD duplex=5 nmol = 33µg

Creative Biogene

User Avatar

MaMaMia

Lvl 5
3y ago

What else can I help you with?

Related Questions

How can one differentiate between transcription and translation in molecular biology and remember which process involves the conversion of DNA to RNA and which involves the conversion of RNA to proteins?

Transcription is the process of converting DNA into RNA, while translation is the process of converting RNA into proteins. To differentiate between the two, remember that transcription involves copying genetic information from DNA to RNA, while translation involves reading the RNA code to build proteins.


Translation is conversion of an RNA message into a what?

Translation is the conversion of an RNA message into a sequence of amino acids, forming a protein.


Rna contains what between the RNa?

RNA contains nucleotides, which are the building blocks of RNA molecules. Nucleotides are connected in a specific sequence to form the RNA molecule.


How do you isololate RNA?

How to isolate mRNA Minou Nowrousian Background: mRNAs (messenger RNAs) comprise only a small percentage of all RNA species in a eukaryotic cell, in Neurospora usually ~ 1-6 % (Lucas et al., 1977; Sturani et al., 1979). For some applications like preparation of a cDNA library, target preparation for microarray hybridizations or Northern blot analysis of weakly expressed genes, enriched mRNA preparations are preferable to total RNA. Enrichment of eukaryotic mRNAs derived from nuclear encoded genes is done by virtue of their poly(A) tail which in most cases is 30-200 nt long. mRNA or poly(A)-RNA preparation consists of three steps: (1) hybridization of poly(A)-containing RNAs to oligo-dT molecules connected to a carrier, (2) washing off nucleic acids which do not bind to oligo-dT


Which one of the following catalyzes the linkage between nucleotides to form mRNA reverse transcriptase tRNA RNA RNA ligase RNA polymeras?

RNA polymerase catalyzes the linkage between nucleotides to form mRNA during the process of transcription. Reverse transcriptase is an enzyme that synthesizes DNA from an RNA template, tRNA is involved in protein synthesis, and RNA ligase is responsible for joining RNA molecules together.


Difference between the DNA and RNA?

the difference is that DNA is a double helix and RNA is a single chain


Why there are no hydrogen bonds present in RNA?

Hydrogen bonds are indeed present in RNA, particularly between complementary bases in the double-stranded regions of RNA molecules, such as between adenine and uracil (or thymine) and between guanine and cytosine. These hydrogen bonds are essential for maintaining the structure and stability of RNA molecules.


State the three main differences between RNA and DNA?

There are three main differences between RNA and DNA: The sugar in RNA is ribose instead of deoxyribose, RNA is generally single-stranded, and RNA contains uracil in place of thymine.


What is the difference between virus and baterias?

Bacteria has both DNA and RNA where as Virus has either DNA or RNA


What are the 30 differences between DNA and RNA?

Comparing DNA and RNA, some key differences include: DNA is double-stranded, while RNA is single-stranded; DNA contains deoxyribose sugar, RNA contains ribose sugar; DNA has thymine base, RNA has uracil base; DNA is found in the nucleus, RNA is found in the cytoplasm; DNA is stable, RNA is less stable; DNA is the genetic material, RNA is involved in protein synthesis. These are just a few of the many distinctions between DNA and RNA.


What is the difference between an amino acid and RNA?

RNA is a polymer of ribonucleic acids. Amino acids are the monomers of proteins.


What are the 3 structural differences between DNA and RNA?

The three main structural differences between DNA and RNA are: DNA is double-stranded, while RNA is single-stranded. DNA contains the sugar deoxyribose, while RNA contains the sugar ribose. DNA contains the base thymine, while RNA contains the base uracil instead.