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Test used for the identification of Staphylococcus aureus?
The coagulase test is commonly used to identify Staphylococcus aureus. This test detects the enzyme coagulase produced by S. aureus, which causes plasma to clot. Positive results indicate the presence of S. aureus, while negative results are obtained for other Staphylococcus species.
What test differentiate staph aureus from other staph species?
The coagulase test is used to differentiate Staphylococcus aureus from other staphylococcal species. S. aureus is coagulase-positive, meaning it can produce the enzyme that causes blood plasma to clot, while other staphylococci, such as Staphylococcus epidermidis and Staphylococcus saprophyticus, are typically coagulase-negative. This test is crucial for accurate identification and appropriate treatment of infections caused by these bacteria.
Does staphylococcus aureus test positive for nitrate reduction?
Staphylococcus aureus is not known to have nitrate reduction capability. Nitrate reduction is a feature commonly associated with bacteria like Escherichia coli and some other enteric bacteria. Staphylococcus aureus typically does not reduce nitrate to nitrite or nitrogen gas.
Is staphylococcus aureus triple sugar iron test positive?
No, Staphylococcus aureus typically produces negative results on the triple sugar iron test as it cannot ferment the sugars present in the medium.
Is staphylococcus aureus negative for the methyl red test?
Yes, Staphylococcus aureus is negative for the methyl red test due to its inability to produce stable acids from glucose fermentation. This distinguishes it from other enteric bacteria that are positive for the methyl red test.
Is staphylococcus aureus positive for gelatin?
Yes it is. This test can be used to differentiate between S. aureus (which is positive) and S. epidermidis (which is negative).
Is staphylococcus aureus methyl red positive?
Staphylococcus aureus is typically methyl red negative. Methyl red test is used to determine the ability of an organism to perform mixed-acid fermentation of glucose, with a positive result indicating a significant drop in pH. Staphylococcus aureus is not known to produce enough acid to yield a positive result with the methyl red test.
What is a coagulase test used for?
The coagulase test is used to differentiate between Staphylococcus aureus (coagulase positive) and other Staphylococcus species (coagulase negative). Staphylococcus aureus is a pathogenic bacterium, while other species are often less virulent. The test involves detecting the ability of the bacterium to coagulate plasma by producing the enzyme coagulase.
What culture media is specific for Staphylococcus aureus?
Mannitol Salt Agar (MSA) is a selective and differential medium that is specific for Staphylococcus aureus. Staphylococcus aureus can ferment mannitol, leading to acid production that changes the pH indicator in the medium, causing yellow colonies to form. Additionally, the high salt concentration in MSA inhibits the growth of other bacteria, allowing for selective isolation of Staphylococcus aureus.
Is coagulase test a microbiological test?
Yes, the coagulase test is a microbiological test used to determine the presence of the enzyme coagulase produced by some Staphylococcus bacteria. It helps differentiate between coagulase-positive Staphylococcus aureus and coagulase-negative staphylococci.
What are the biochemical tests to confirm staphylococcus?
Streptococci are not differentiated by biochemical tests, but rather by hemolysis reaction (beta Strep or alpha Strep) and sero-group (as in Group E Strep).Further, streptococcus is also identified by thr following tests:1. Coagulase.2. Haemolysin (It lyses 4% RBC suspension)3. Fibrinolysin (It can dissolve a fibrin clot)4. Strp. Pneumoniae also gives a positive bile solubility test.5. It ferments Inulin (A sugar)
Is citrate test results for Staphylococcus aureus?
No, Catalase +ve, Coagulase +ve, Gram + cocci. Citrate test is usually used to differentiate members of the Family Enterobacteriaceae. Check Bergeys systematic bacteriology. 1980
