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Biochemical test for identification of gram positive bacteria?

the answer i would like to give. there are many biochemical tests for detection of various types of bacteria. 1. carbohydrate utilization tests (sugar test). - lactose , sucrose , mannitol , maltose , xylose and dextrose. 2. imvic test. it includes indole test , methyl red test , voges proskuer test , citrate utilization test. 3. nitrate reduction test. 4. urease test 5. triple sugar iron test. 6. lead acetate test. 7. mannitol motility test 8. O-F (oxidation - fermentation) test 9. aminoacid degradation test we can detect enterobacteriaecae group & other group of bacilli according to their charactristics by comparing with standard table.


How do mannitol-salt agar tests work?

Mannitol salt agar supports growth of organisms that can grow in a high salt concentration, particularly Staphylococcus species and halophiles. The phenol red pH indicator in the agar will also let you know whether or not the bacterium you streaked ferments mannitol by changing to a yellow color if fermentation has occured.


What are the three sugars in triple sugar iron agar?

The triple sugar iron agar is a differential medium. The three sugars it contains are:lactose (1%)sucrose (1%)glucose (0.1%)


What will grow on mannitol salt agar besides staph?

Mannitol Salt Agar (MSA) is a selective/differential media that inhibits the growth of gram negative bacteria. Only a few bacteria can tolerate the 7.5% NaCl found within this media. The high salt content selects for Staphylococcus and a few salt tolerant enterococci. The presence of the sugar mannitol gives this media its differential properties. When mannitol is fermented (utilized by the bacteria) microorganisms release acidic byproducts that change the pH of the surrounding media. The pH change is indicated by a change in the pH indicator (phenol red) from pink to yellow.


Which bacteria is NLF oxidase negative motile citrate and urease positive and TSI alkali nochange?

The bacteria described is likely to be Proteus species. Proteus is negative for oxidase, motile, and positive for citrate utilization, urease production, and TSI (triple sugar iron) test showing an alkaline/no change reaction. These characteristics are commonly associated with Proteus species.

Related Questions

What are the biochemical tests to confirm staphylococcus?

Streptococci are not differentiated by biochemical tests, but rather by hemolysis reaction (beta Strep or alpha Strep) and sero-group (as in Group E Strep).Further, streptococcus is also identified by thr following tests:1. Coagulase.2. Haemolysin (It lyses 4% RBC suspension)3. Fibrinolysin (It can dissolve a fibrin clot)4. Strp. Pneumoniae also gives a positive bile solubility test.5. It ferments Inulin (A sugar)


What is the reaction of staphylococcus in TSI agar?

Staphylococcus typically shows no reaction in Triple Sugar Iron (TSI) agar due to its inability to ferment lactose or produce hydrogen sulfide. Staphylococcus colonies on TSI agar appear as small, round, and colorless colonies without any color changes in the medium.


What are pathogenic bacteria found in sugar?

Pathogenic bacteria that can be found in sugar include Salmonella, Escherichia coli (E. coli), and Staphylococcus aureus. These bacteria can contaminate sugar during processing or handling, especially if proper hygiene practices are not followed. While sugar itself is not a conducive environment for bacterial growth, contaminated sugar can pose health risks if consumed. Proper storage and handling are essential to prevent contamination.


What is a triple-triple in coffee?

It means 3 shots of sugar and cream


Biochemical test for identification of gram positive bacteria?

the answer i would like to give. there are many biochemical tests for detection of various types of bacteria. 1. carbohydrate utilization tests (sugar test). - lactose , sucrose , mannitol , maltose , xylose and dextrose. 2. imvic test. it includes indole test , methyl red test , voges proskuer test , citrate utilization test. 3. nitrate reduction test. 4. urease test 5. triple sugar iron test. 6. lead acetate test. 7. mannitol motility test 8. O-F (oxidation - fermentation) test 9. aminoacid degradation test we can detect enterobacteriaecae group & other group of bacilli according to their charactristics by comparing with standard table.


How do mannitol-salt agar tests work?

Mannitol salt agar supports growth of organisms that can grow in a high salt concentration, particularly Staphylococcus species and halophiles. The phenol red pH indicator in the agar will also let you know whether or not the bacterium you streaked ferments mannitol by changing to a yellow color if fermentation has occured.


When sugar dissolves es in water is the change in entropy positive or negative?

When sugar dissolves in water, the change in entropy is generally positive. This is because the sugar molecules become more dispersed in the solvent, increasing the disorder or randomness of the system.


What are the three sugars in triple sugar iron agar?

The triple sugar iron agar is a differential medium. The three sugars it contains are:lactose (1%)sucrose (1%)glucose (0.1%)


Why is Msa argar a selective medium?

Mannitol Salt Agar is selective for staphylococci as the high salt (sodium chloride) levels prohibit most other bacteria from surviving and it is differential as Staphylococci ferment mannitol, producing acid, lowering the pH and turning the media yellow. The development of yellow media presumes the bacteria to be pathogenic Staphylococcus (usually S. aureus). From A Photographic Atlas for the Microbiology Laboratory by Leboffe and Pierce.


What liquor has the highest sugar content?

Liqueurs, such as triple sec or amaretto, typically have the highest sugar content among liquors.


How much sugar does a margarita contain?

It depends on your Margarita recipe but , assuming that you make your drink form Ice, tequilalime juice and triple-sec, then the only sugar will be from the triple-sec and to find out how much that is you need to look at the bottle.


What will grow on mannitol salt agar besides staph?

Mannitol Salt Agar (MSA) is a selective/differential media that inhibits the growth of gram negative bacteria. Only a few bacteria can tolerate the 7.5% NaCl found within this media. The high salt content selects for Staphylococcus and a few salt tolerant enterococci. The presence of the sugar mannitol gives this media its differential properties. When mannitol is fermented (utilized by the bacteria) microorganisms release acidic byproducts that change the pH of the surrounding media. The pH change is indicated by a change in the pH indicator (phenol red) from pink to yellow.