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Due to years of anti-toxin abuse, multidrug resistance is currently the tenet instead of the special case among safe microorganisms. This circumstance has to a great extent happened through the consecutive utilization of numerous distinctive anti-microbials.
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What type of gene is used to distinguish bacteria that carry a plasmid containing foreign DNA from those that don' t?
The plasmid that contains foreign DNA is engineered to also carry an antibiotic resistance gene. This antibiotic resistance gene codes for a protein that is able to inactivate an antibiotic thus keeping the cell alive. In the absence of the antibiotic resistance gene, the cells would not survive when exposed to an antibiotic. After transfection (the process of inserting the plasmid carrying the foreign gene into cells), the cells are gown in media containing an antibiotic. Cells that contain the plasmid (and therefore contain the antibiotic resistance gene) are able to survive in this medium. Cells that do not contain the plasmid (and therefore lack the antibiotic resistance gene) do not survive in this medium. The process described above is called selection
True or false cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
false
What is an extra loop of DNA that contains antibiotic resistance what gene?
Plasmid
A gene for antibiotic resistance may be used as a making it possible to identify a transformed cell?
plasmids take over an eat
Why are plasmids pieces of DNA?
i think each plasmid piece codes for a specific function. for example antibiotic resistance shown by antibiotic sensitive cell after a piece of plasmid that is antibiotic resistant gene recombine with cell DNA.
Antibiotic in gene cloning?
If antibiotic resistance is added to the gene being cloned, antibiotics can be used to isolate the transformed bacteria (ones with the gene being cloned) by killing off all non-transformed bacteria, that don't have the antibiotic resistance. There is a chance that the non-transformed bacteria can mutate to develop antibiotic resistance.
What can gene for antibiotic resistance be used for?
genetic marker
What type of gene is used to distinguish bacteria that carry a plasmid containing foreign DNA from those that don' t?
The plasmid that contains foreign DNA is engineered to also carry an antibiotic resistance gene. This antibiotic resistance gene codes for a protein that is able to inactivate an antibiotic thus keeping the cell alive. In the absence of the antibiotic resistance gene, the cells would not survive when exposed to an antibiotic. After transfection (the process of inserting the plasmid carrying the foreign gene into cells), the cells are gown in media containing an antibiotic. Cells that contain the plasmid (and therefore contain the antibiotic resistance gene) are able to survive in this medium. Cells that do not contain the plasmid (and therefore lack the antibiotic resistance gene) do not survive in this medium. The process described above is called selection
A gene for antibiotic resistance may be used as?
Genetic Marker
Is it true cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
false
True or false cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
false
What is an extra loop of DNA that contains antibiotic resistance what gene?
Plasmid
How can specialized transduction contribute to the transfer of antibiotic resistance genes in a bacterial population?
The prophage takes an antibiotic resistance gene with it and is packaged with the newly synthesized viral DNA.
What one plate would you first inspect to conclude that the transformation occurred successfully Why?
If you transform bacteria with a plasmid containing a selection marker (such as an antibiotic resistance gene) and plate the transformed bacteria on a plate suited for selecting for plasmid-containing bacteria (such as a plate containing an antibiotic that only those bacteria with antibiotic resistance can survive), then simply inspecting whether colonies are present on the plate will suffice in determining whether the transformation succeeded. If no colonies are found, that means no bacteria got the antibiotic resistance gene on the plasmid and the transformation was unsuccessful. If some colonies are found, that means some bacteria contain the plamis containing the antibiotic resistance gene and those colonies can the transformation was successful.
A gene for antibiotic resistance may be used as a making it possible to identify a transformed cell?
plasmids take over an eat
Why are plasmids pieces of DNA?
i think each plasmid piece codes for a specific function. for example antibiotic resistance shown by antibiotic sensitive cell after a piece of plasmid that is antibiotic resistant gene recombine with cell DNA.
The use of an antibiotic resistance gene on a plasmid used in genetic engineering makes?
direct selection possible
