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What is applied during the differential stage of gram staining?
Wiki User
∙ 9y ago
In all bacteria that are Gram stained all the steps are used. It isn't until the end that you will know if they are Gram+ or Gram-. In Gram staining, all bacterial cells take up the crystal violet.
Iodine is then added; it acts as a mordant, a chemical that helps retain the stain in certain cells.
Those structures that cannot retain crystal violet are decolorized with 95% ethanol or an ethanol- acetone solution, rinsed, and subsequently stained (counterstained) with safranin (red).
Gram+ will be violet while Gram- will be red. The thick cell walls of Gram-positive bacteria retain such stains as the crystal violet-iodine dye in the cytoplasm. Gram- will not.
Wiki User
∙ 9y ago
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