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What is difference between chromophore and auxochrome?
Chromophores are responsible for giving dyes their color by absorbing certain wavelengths of visible light and transmitting or reflecting others.Auxochromes modify the ability of a chromophore to absorb light. They often result in the deepening and intensifying of the color of compounds
Is methylene blue a chromophore?
Nope - methylene blue is a stain. You need liquid indicator like phenolphthalein that responds to pH changes.
What is the mechanism involved in Liebermann- Burchard test?
When conc. sulfuric acid is added to cholesterol, a water molecule is removed from C3 of cholesterol molecule, and it is oxidised to form3,5-cholestadiene.This product is converted to a polymer containing a chromophore which results in the green colouration indicating a positive test.
How is a protein tagged to a fluorescent protein?
The most common protein that is used as a 'tag' for other proteins is GFP. In order to do this, the GFP gene sequence is simply added at the end of the gene sequence for the target protein. GFP is particularly useful for this because it contains its own chromophore, and needs nothing else except the gene sequence to create its own light. This makes possible easy tracking of certain proteins without necessarily killing the tester animal.
Chemical makeup of bleach?
Colored substances contain molecules with chromophores, areas of the molecule that have double bonds between carbon atoms or oxygen atoms. A good example is beta carotene, and that section goes into more detail on how molecules become colored. Bleaches attack these chromophores in one of two ways. Oxidizing bleaches like sodium hypochlorite break the molecules at the double bond. This results in either a shorter molecule that does not absorb visible light, or a molecule whose chromophore is either shorter or non-existant. A shorter chromophore will absorb light of a shorter wavelength than visible light (such as ultraviolet light), and so does not appear colored. Reducing bleaches such as lemon juice (in combination with sunlight) or sulfur dioxide, convert the double bonds in the chromophore into single bonds, eliminating its ability to absorb visible light. Sometimes the reaction is reversible, where oxygen in the air reacts with the molecule to repair the chromophore, and the stain returns. Another chlorine bleach often used is sodium dichloroisocyanurate. Oxygen bleaches also work by releasing oxygen. Hydrogen peroxide is the active ingredient, either as itself, or as a product of reacting another ingredient with water to release hydrogen peroxide. Oxygen bleaches such as sodium carbonate peroxide (also called sodium percarbonate), sodium peroxide, or sodium perborate are made by reacting molecules with hydrogen peroxide. When the result is added to water, the hydrogen peroxide is released. Borax also works by releasing hydrogen peroxide into the water. Most oxygen bleaches work best in hot water. Additives such as tetra acetyl ethylene diamine allow the hydrogen peroxide to work in warm water (50° C).
What is an auxochrome?
An auxochrome is any atom or group which, when added to a chromophore, causes a bathochromic shift in its spectrum.
What is difference between chromophore and auxochrome?
Chromophores are responsible for giving dyes their color by absorbing certain wavelengths of visible light and transmitting or reflecting others.Auxochromes modify the ability of a chromophore to absorb light. They often result in the deepening and intensifying of the color of compounds
What effect does a chromophore have on benzene?
It might be that benzene, a non-polar hydrocarbon solvent, will have an effect on a chromophore. A chromophore is, generally speaking, the portion of a molecule that affects the way it absorbs and reflects light, thereby giving it color. Benzne, C6H6, might react with the chromophore to alter it and change the color of a substance, but the substance would have to be specified before any attempt was made to predict the affects of benzene on its color.
Which ion carries the chromophore in an acidic dye?
The negative ion
What is a clomophore?
A chromophore is part of a molecule. This part is what gives the molecule its color by absorbing and reflecting light.
Can colorimetry be used to test concentration of citric acid?
It can be if you can convert the citric acid to a chromophore, i.e., something that is colored. Otherwise, you cannot.
Is methylene blue a chromophore?
Nope - methylene blue is a stain. You need liquid indicator like phenolphthalein that responds to pH changes.
What is the process bleaching?
An oxidizing bleach works by breaking the chemical bonds of a chromophore (part of a molecule that has color). This changes the molecule so that it either has no color or else reflects color outside the visible spectrum. A reducing bleach works by changing the double bonds of a chromophore into single bonds. This alters the optical properties of the molecule, making it colorless.
What is bleaching process?
An oxidizing bleach works by breaking the chemical bonds of a chromophore (part of a molecule that has color). This changes the molecule so that it either has no color or else reflects color outside the visible spectrum. A reducing bleach works by changing the double bonds of a chromophore into single bonds. This alters the optical properties of the molecule, making it colorless.
What reacts to light to start the process of transduction?
Retinal reacts to light to start the process of transduction. Retinal is a polyene chromophore, and is sometimes referred to as retinaldehyde.
What has the author Melanie Barfels written?
Melanie Barfels has written: 'Chromophore imaging with an optimized prism-mirror-prism energy filter designed for an electron microscope'
What is the difference between chromophore and fluorophore?
1. chromophore is an isolated covalently bonded group which characteristically absorbs in the visible range of electromagnetic radiation. But fluorophore is any group capable of absorbing energy and emitting radiation with a longer wavelength. 2. Chromophores may have a overlapping ground singlet and excited singlet state but a fluorophore must have non overlapping S0 and S1 orbitals. 3. Chromophores can't emit energy but fluorophores can. 4. Chromophores are of 2 main types, extended π bond system and metal complex chromophore. Fluorophores are of 2 types, extrinsic and intrinsic. 5. Chromophores can't be quenched but fluorophores can. 6. Chromophores' absorption is not temperature dependent but fluorophores' fluorescing capacity is temperature dependent.
