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When conc. sulfuric acid is added to cholesterol, a water molecule is removed from C3 of cholesterol molecule, and it is oxidised to form3,5-cholestadiene.This product is converted to a polymer containing a chromophore which results in the green colouration indicating a positive test.
Wiki User
∙ 16y ago
Wiki User
∙ 10y agoIt is a reaction between acetic anhydride (mixed with sulfuric acid) and the hydroxyl group of cholesterol; a green color is obtained in the presence of cholesterol. The reaction is a colorimetric test.
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