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It is a standard method defined in the M27-A2 documentation from the National Committee for Clinical Laboratory Standards. The method is for antifungal susceptibility testing and determines the minimum inhibitory concentration, which is the concentration of the antifungal in a dilution series distributed in culture broth wells, where there is no growth of the tested organism.

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6mo ago

The broth microdilution method CLSI M27 A2 is a standardized procedure used to determine the minimum inhibitory concentration (MIC) of antifungal drugs against fungi. It involves diluting the antifungal drug in a series of broth tubes containing the fungal isolate and incubating them for a specified period. The MIC is defined as the lowest concentration of the drug that inhibits visible growth of the fungus.

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Q: What is broth microdilution method CLSI M27 A2?
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