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What happens to protein charge if pH value becomes lower than pi in 2 dimensional gel electrophoresis?
If the pH value becomes lower than the protein's isoelectric point (pI) in 2D gel electrophoresis, the protein will acquire a net positive charge due to the excess of protons. This will cause the protein to move towards the cathode during electrophoresis.
What is the main difference between protein electrophoresis and nucleic acid electrophoresis?
There are many similarities and differences between protein and DNA electrophoresis.Similarities:PAGE protein and DNA electrophoresis both cause separation by size, creating bands that are viewed by the scientist or a machine. The smallest segments more the fastest due to less friction with the surface of their medium or equipment.The movement of charges through the medium is what causes the DNA or proteins to move. Electrons move from the negative to positive end of the gel or capillary tube.Differences:In PAGE protein electrophoresis, a polyacrylamide gel is used to prevent convection from altering the movement of the proteins. If the proteins are charged, and there is a worry that the charge will affect the mobility of the protein segments, 1% SDS can be added to get rid of the mass/charge issue. This way, only the mass of the segment determines how far it moves. In DNA capillary electrophoresis, the size of the capillary is so small that it does not have room for convection to occur (it is only 20-50 microns wide). Thus, there is no medium in the capillary but DNA itself.In protein electrophoresis, the proteins are often dyed so their movement can be viewed with the naked eye, or a machine. With DNA capillary electrophoresis, DNA strands are made through DNA replication with dNTPs that are fluorescently labeled for the different nucleotides. Each base is labeled a different color. A fine laser lights up the DNA strand in the capillary tube and reads what color fluoresces. This is how the nucleotide is identified.Protein PAGE electrophoresis is used to determine the purity of a protein sample. It can also be used to see how large the chains are that make up a multi-chain protein if a denaturing agent is added. DNA electrophoresis is used to get the order of nucleotides in a DNA sequence. It is done by chopping the DNA sequence into many smaller bits and sequencing them, then putting them back together by lining them up according to sequence overlaps. This is called the "shotgun" method. Protein electrophoresis can figure out the order of about 15-20 amino acids by a similar method, but DNA electrophoresis can get up to 1000 nucleotides (~300 amino acids). DNA electrophoresis is limited by the low probability that the DNA sequence would be cut into a segment greater than 1000 nucleotides.
How sample overload effect electrophoresis?
Sample overload will result in the formation of a streak rather than separate bands.. And confuses with the results!!!! And moreover it will get you a good name from your boss" can't even run a proper gel!!!(lol)"...
What problems might arise if the agarose wasn't dissolved completely or if there were air bubbles in the gel?
Incomplete dissolution of agarose can lead to uneven gel density, affecting electrophoresis results. Air bubbles in the gel can cause irregular migration of DNA bands, distorting the final outcome by affecting the separation pattern. Both scenarios can compromise the accuracy and reproducibility of the experiment.
What are three mechanisms that cause allele frequency differences in a population?
Mutation, migration, and genetic drift
What happens to protein charge if pH value becomes lower than pi in 2 dimensional gel electrophoresis?
If the pH value becomes lower than the protein's isoelectric point (pI) in 2D gel electrophoresis, the protein will acquire a net positive charge due to the excess of protons. This will cause the protein to move towards the cathode during electrophoresis.
Why Electrophoresis power supply results in longer gel run time?
There are several factors that control the rate at which a sample moves or migrates in a gel. One of those factors is electric power supply. The larger the voltage applied, the faster the migration. However, there is an upper limit to how much voltage can be applied. If the voltage is too high, it will cause heating in the electrophoresis module and this is turn will negatively affect the integrity of the gel.
What is the main difference between protein electrophoresis and nucleic acid electrophoresis?
There are many similarities and differences between protein and DNA electrophoresis.Similarities:PAGE protein and DNA electrophoresis both cause separation by size, creating bands that are viewed by the scientist or a machine. The smallest segments more the fastest due to less friction with the surface of their medium or equipment.The movement of charges through the medium is what causes the DNA or proteins to move. Electrons move from the negative to positive end of the gel or capillary tube.Differences:In PAGE protein electrophoresis, a polyacrylamide gel is used to prevent convection from altering the movement of the proteins. If the proteins are charged, and there is a worry that the charge will affect the mobility of the protein segments, 1% SDS can be added to get rid of the mass/charge issue. This way, only the mass of the segment determines how far it moves. In DNA capillary electrophoresis, the size of the capillary is so small that it does not have room for convection to occur (it is only 20-50 microns wide). Thus, there is no medium in the capillary but DNA itself.In protein electrophoresis, the proteins are often dyed so their movement can be viewed with the naked eye, or a machine. With DNA capillary electrophoresis, DNA strands are made through DNA replication with dNTPs that are fluorescently labeled for the different nucleotides. Each base is labeled a different color. A fine laser lights up the DNA strand in the capillary tube and reads what color fluoresces. This is how the nucleotide is identified.Protein PAGE electrophoresis is used to determine the purity of a protein sample. It can also be used to see how large the chains are that make up a multi-chain protein if a denaturing agent is added. DNA electrophoresis is used to get the order of nucleotides in a DNA sequence. It is done by chopping the DNA sequence into many smaller bits and sequencing them, then putting them back together by lining them up according to sequence overlaps. This is called the "shotgun" method. Protein electrophoresis can figure out the order of about 15-20 amino acids by a similar method, but DNA electrophoresis can get up to 1000 nucleotides (~300 amino acids). DNA electrophoresis is limited by the low probability that the DNA sequence would be cut into a segment greater than 1000 nucleotides.
What was the cause of Indian migration to America?
what are the causes of the migration of the navajos
What were cause of the great migration?
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Which was not a major cause for Bantu migration?
Trade
What methods are commonly used to purify proteins?
Common methods used to purify proteins include chromatography, electrophoresis, and precipitation. Chromatography separates proteins based on their size, charge, or affinity for a specific ligand. Electrophoresis separates proteins based on their charge and size. Precipitation involves adding a reagent to the protein solution to cause the proteins to come out of solution and form a solid precipitate, which can then be separated from the rest of the solution.
What nutrients help hair grow faster?
No nutrients helps hair grow faster, but lack of nutrients may cause your hair to grow slower. Generally, your hair cells need the same nutrients your normal cells need, plus more protein as hair is covered with keratin, a protein combination.
Can herpes cause high protein in blood?
Herpes does not cause high protein in the blood.
Why are alkaline conditions used in electrophoresis?
A requirement of the gel is that it is neutral and that included the absence of charged impurities. If the gel contains any charged groups then an effect known as electroendosmosis will take place. As these charged groups are immobilised onto the gel they cause a solvent flow towards one of the electrodes, usually the cathode (negative) and thus in opposition to the sample flow. This is obviously undesirable as this will slow down and may distort the migration of the samples reducing resolution which can compromise quantitative accuracy and precision. Operation at extremes of pH to minimize these problems is useful in special cases but is not a general strategy for protein separations
What is the name of the disease caused by not eating enough protein?
A lack of protein does not cause an eating disorder. Instead, that can cause anemia.
How does the economy cause desertification?
livestock size and migration of livestock
