0
Simply, smaller proteins migrating through the holes tunneled in the gel.
Wiki User
∙ 12y ago
Add your answer:
Earn +20 pts
What is the main difference between protein electrophoresis and nucleic acid electrophoresis?
There are many similarities and differences between protein and DNA electrophoresis.Similarities:PAGE protein and DNA electrophoresis both cause separation by size, creating bands that are viewed by the scientist or a machine. The smallest segments more the fastest due to less friction with the surface of their medium or equipment.The movement of charges through the medium is what causes the DNA or proteins to move. Electrons move from the negative to positive end of the gel or capillary tube.Differences:In PAGE protein electrophoresis, a polyacrylamide gel is used to prevent convection from altering the movement of the proteins. If the proteins are charged, and there is a worry that the charge will affect the mobility of the protein segments, 1% SDS can be added to get rid of the mass/charge issue. This way, only the mass of the segment determines how far it moves. In DNA capillary electrophoresis, the size of the capillary is so small that it does not have room for convection to occur (it is only 20-50 microns wide). Thus, there is no medium in the capillary but DNA itself.In protein electrophoresis, the proteins are often dyed so their movement can be viewed with the naked eye, or a machine. With DNA capillary electrophoresis, DNA strands are made through DNA replication with dNTPs that are fluorescently labeled for the different nucleotides. Each base is labeled a different color. A fine laser lights up the DNA strand in the capillary tube and reads what color fluoresces. This is how the nucleotide is identified.Protein PAGE electrophoresis is used to determine the purity of a protein sample. It can also be used to see how large the chains are that make up a multi-chain protein if a denaturing agent is added. DNA electrophoresis is used to get the order of nucleotides in a DNA sequence. It is done by chopping the DNA sequence into many smaller bits and sequencing them, then putting them back together by lining them up according to sequence overlaps. This is called the "shotgun" method. Protein electrophoresis can figure out the order of about 15-20 amino acids by a similar method, but DNA electrophoresis can get up to 1000 nucleotides (~300 amino acids). DNA electrophoresis is limited by the low probability that the DNA sequence would be cut into a segment greater than 1000 nucleotides.
Do protein cause humans to get fat?
no
What is the function of agarose gel electrophoresis?
Agarose gel electrophoresis is for determining the size of a piece of RNA or DNA. It can be used to determine the culprit of a crime, relatives, even the cause of some diseases, like Mad Cow.
How can a protein lose its tertiary structure?
affected by temperature or pH cause the protein denature .
Can a mutation cause a protein to be overexpressed?
Yes
Why Electrophoresis power supply results in longer gel run time?
There are several factors that control the rate at which a sample moves or migrates in a gel. One of those factors is electric power supply. The larger the voltage applied, the faster the migration. However, there is an upper limit to how much voltage can be applied. If the voltage is too high, it will cause heating in the electrophoresis module and this is turn will negatively affect the integrity of the gel.
What is the main difference between protein electrophoresis and nucleic acid electrophoresis?
There are many similarities and differences between protein and DNA electrophoresis.Similarities:PAGE protein and DNA electrophoresis both cause separation by size, creating bands that are viewed by the scientist or a machine. The smallest segments more the fastest due to less friction with the surface of their medium or equipment.The movement of charges through the medium is what causes the DNA or proteins to move. Electrons move from the negative to positive end of the gel or capillary tube.Differences:In PAGE protein electrophoresis, a polyacrylamide gel is used to prevent convection from altering the movement of the proteins. If the proteins are charged, and there is a worry that the charge will affect the mobility of the protein segments, 1% SDS can be added to get rid of the mass/charge issue. This way, only the mass of the segment determines how far it moves. In DNA capillary electrophoresis, the size of the capillary is so small that it does not have room for convection to occur (it is only 20-50 microns wide). Thus, there is no medium in the capillary but DNA itself.In protein electrophoresis, the proteins are often dyed so their movement can be viewed with the naked eye, or a machine. With DNA capillary electrophoresis, DNA strands are made through DNA replication with dNTPs that are fluorescently labeled for the different nucleotides. Each base is labeled a different color. A fine laser lights up the DNA strand in the capillary tube and reads what color fluoresces. This is how the nucleotide is identified.Protein PAGE electrophoresis is used to determine the purity of a protein sample. It can also be used to see how large the chains are that make up a multi-chain protein if a denaturing agent is added. DNA electrophoresis is used to get the order of nucleotides in a DNA sequence. It is done by chopping the DNA sequence into many smaller bits and sequencing them, then putting them back together by lining them up according to sequence overlaps. This is called the "shotgun" method. Protein electrophoresis can figure out the order of about 15-20 amino acids by a similar method, but DNA electrophoresis can get up to 1000 nucleotides (~300 amino acids). DNA electrophoresis is limited by the low probability that the DNA sequence would be cut into a segment greater than 1000 nucleotides.
What was the cause of Indian migration to America?
what are the causes of the migration of the navajos
What were cause of the great migration?
== == == == == ==
Which was not a major cause for Bantu migration?
Trade
What nutrients help hair grow faster?
No nutrients helps hair grow faster, but lack of nutrients may cause your hair to grow slower. Generally, your hair cells need the same nutrients your normal cells need, plus more protein as hair is covered with keratin, a protein combination.
Can herpes cause high protein in blood?
Herpes does not cause high protein in the blood.
How does the economy cause desertification?
livestock size and migration of livestock
What are the cause of animal migration?
Starvation, harsh climates, and fires.
What is the name of the disease caused by not eating enough protein?
A lack of protein does not cause an eating disorder. Instead, that can cause anemia.
Is protein a mineral?
Is Mineral A Protein would be a real question cause MINERALS ARE A PROTEIN!
When does mass cause an object to fall faster?
Mass does not cause an object to fall faster.
