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What would happen to eukaryotic cells over repeated cell divisions if they didn't have telomeres?
the nucleotides at the terminal ends will be removed due to exonuclease activity of DNase present in the cells of the eukaryotes.and therefore the DNA strand will get shortened.
What is the enzyme that opens up DNA?
restriction endonuclease and exonuclease
What is a linker DNA?
Linker DNA links adjacent nucleosome core particles to each other. They then resemble beads on a string. These linker DNAs are readily removed through exonuclease hydrolysis.
What are the similarities between DNA polymerase and RNA polymerase?
Both RNA and DNA form in the same manner. They add bases to the 3' end of the base to form a polymer.
When does a cell make sure DNA has been copied correctly before continuing in the cell cycle?
DNA is copied (or replicated) by an enzyme called DNA polymerase. This enzyme generally operates in the 3' to 5' direction. However, this enzyme also has a 5' to 3' exonuclease activity, which enables it to remove certain wrong bases and insert correct ones. This ensure that the DNA is copied correctly
What is the difference between DNA polymerase III and DNA polymerase I?
pol 1 - exonuclease activity pol 2 - dna repair pol 3 - primary replication enzyme
What is the difference between an endonuclease and an exonuclease?
an exonuclease cleaves nucleotides from the end of a polynucleotide chain whereas an endonuclease cleaves nucleotides from within a polynucleotide chain
What would happen to eukaryotic cells over repeated cell divisions if they didn't have telomeres?
the nucleotides at the terminal ends will be removed due to exonuclease activity of DNase present in the cells of the eukaryotes.and therefore the DNA strand will get shortened.
What is the enzyme that opens up DNA?
restriction endonuclease and exonuclease
What type of biological molecule is an exonuclease?
Proteins that uses the energy of NTP hydrolysis to separate the DNA strands.
Difference Between 3' to 5' and 5' to 3' Exonuclease Activity?
3'-5' is a characteristic feature of DNA-polymerase I. This activity is meant to repair any misparing mistakes that the enzyme may commit during the synthesis, in which the enzyme would reverse its direction by ONE NUCLEOTIDE and excised the mistakenly added nucleotide, the enzyme acts at the phosphodiester bond at the 5 prime. Whereas the 5'-3' exonuclease activity is an also repair strategy exercised by the DNA polymerase I. However, in this case the polymerase would move in the forwards direction and excise the miss-matched nucleotides at any position regardless with one nucleotide far or so many. This mechanism of repair is well documented in case UV-mutation.
What is a linker DNA?
Linker DNA links adjacent nucleosome core particles to each other. They then resemble beads on a string. These linker DNAs are readily removed through exonuclease hydrolysis.
What is is a linker?
The DNA found between nucleosomes on chromatin; since it is not complexed to proteins as strongly as other forms of dna, it is accessible to exonuclease hydrolysis.
Why do prokaryotic chromosomes not have telomeres?
because it has its own modification and restriction system which prevents its own DNA from degradation as telomeres are mainly responsible to protect the DNA from exonuclease activity of DNase.
What are the similarities between DNA polymerase and RNA polymerase?
Both RNA and DNA form in the same manner. They add bases to the 3' end of the base to form a polymer.
How can one differentiate between single strand DNA and double strand DNA?
One can differentiate between single-strand DNA and double-strand DNA by treating with exonuclease I which specifically digests only ssDNA. Note. For verification, the products have to be run on polyacrylamide gels with appropriate controls. Hope this helps One can differentiate between single-strand DNA and double-strand DNA by treating with exonuclease I which specifically digests only ssDNA. Note. For verification, the products have to be run on polyacrylamide gels with appropriate controls. Hope this helps
What activity is activity without oxygen?
"anaerobic" activity is activity without oxygen.
