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Configuration of the GFP inside bacterial cells?
Inside bacterial cells, the green fluorescent protein (GFP) is typically encoded by a gene that can be introduced into the bacterial genome or expressed on a plasmid. The gene consists of coding sequences that allow the production of the GFP protein, which fluoresces green when exposed to specific wavelengths of light. The gene is regulated by bacterial promoters and terminators to control its expression level. The GFP protein is then synthesized within the bacterial cell and can be visualized using fluorescence microscopy or other techniques.
Why GFP colonies are observed in the plate with pQE30 and GFP construct?
Go to http://www.tsienlab.ucsd.edu/Images.htm you will find bacterial colonies will and can express functional fluorescent proteins. As for the plasmid pQE30, it looks to fit into the category of expression at this level.
Which organelles inside bacteria make green fluorescent protein?
Organelles inside bacteria do not 'make' GFP. GFP can however be expressed in bacteria by adding the coding sequence for GFP to their genome. This can be done by using a plasmid virus containing the GFP gene sequence. When combined with the plasmid, a very small number of bacteria will take up the plasmid's genome into their own. These bacteria can be isolated by using the 'transform or die' mechanism. Bacteria that do not contain the plasmid are preferentially killed by adding an antibody to the agar. The normal bacteria will not be resistant to this, and will die, but the bacteria which have taken up the plasmid into their genome will also contain an antibody resistance gene. This allows them to live in the presence of the antibody. Since GFP was also added to these bacteria's genome, they will fluoresce.
How is a protein tagged to a fluorescent protein?
The most common protein that is used as a 'tag' for other proteins is GFP. In order to do this, the GFP gene sequence is simply added at the end of the gene sequence for the target protein. GFP is particularly useful for this because it contains its own chromophore, and needs nothing else except the gene sequence to create its own light. This makes possible easy tracking of certain proteins without necessarily killing the tester animal.
Why did Osamu Shimomura win The Nobel Prize in Chemistry in 2008?
The Nobel Prize in Chemistry 2008 was awarded jointly to Osamu Shimomura, Martin Chalfie and Roger Y. Tsien for the discovery and development of the green fluorescent protein, GFP.
Is luciferin and gfp the same thing?
No.
What is mw of gfp?
Aequorea GFP is a protein of 238 amino acids with a molecular weight of 27 or 30 kDa. Source: http://www.cryst.bbk.ac.uk/PPS2/projects/jonda/intro.htm
Can GFP be observed under UV light?
Yes, but it will not be green :)
Configuration of the GFP inside bacterial cells?
Inside bacterial cells, the green fluorescent protein (GFP) is typically encoded by a gene that can be introduced into the bacterial genome or expressed on a plasmid. The gene consists of coding sequences that allow the production of the GFP protein, which fluoresces green when exposed to specific wavelengths of light. The gene is regulated by bacterial promoters and terminators to control its expression level. The GFP protein is then synthesized within the bacterial cell and can be visualized using fluorescence microscopy or other techniques.
Why GFP colonies are observed in the plate with pQE30 and GFP construct?
Go to http://www.tsienlab.ucsd.edu/Images.htm you will find bacterial colonies will and can express functional fluorescent proteins. As for the plasmid pQE30, it looks to fit into the category of expression at this level.
Which organelles inside bacteria make green fluorescent protein?
Organelles inside bacteria do not 'make' GFP. GFP can however be expressed in bacteria by adding the coding sequence for GFP to their genome. This can be done by using a plasmid virus containing the GFP gene sequence. When combined with the plasmid, a very small number of bacteria will take up the plasmid's genome into their own. These bacteria can be isolated by using the 'transform or die' mechanism. Bacteria that do not contain the plasmid are preferentially killed by adding an antibody to the agar. The normal bacteria will not be resistant to this, and will die, but the bacteria which have taken up the plasmid into their genome will also contain an antibody resistance gene. This allows them to live in the presence of the antibody. Since GFP was also added to these bacteria's genome, they will fluoresce.
Where can you download software for Fission Film strip scanner Model GFP-001?
From punjabu rap
What are the two possible sources of fluorescence within an Ecol colony when exposed to UV light?
pGLO and GFP
How is a protein tagged to a fluorescent protein?
The most common protein that is used as a 'tag' for other proteins is GFP. In order to do this, the GFP gene sequence is simply added at the end of the gene sequence for the target protein. GFP is particularly useful for this because it contains its own chromophore, and needs nothing else except the gene sequence to create its own light. This makes possible easy tracking of certain proteins without necessarily killing the tester animal.
What part did Sturmbannfuhrer Ludwig Kessler play within the Gestapo?
He was a member of the (GFP) Geheime Feldpolizei. Secret field Police
What are the gfp gus lines of arabidopsis thaliana?
this is awkward. defs not geometry mate. line refers to the "strain" of plant. try plant genetics??
How can genetic engineering be used to make a transgenic tobacco plant that glows in the dark?
Agrobacterium-mediated transformation using a plasmid containing a gene for GFP
