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What is glucose oxidase peroxidase aminophenazone phenol?
Navdeepgirn
Its a reagent used to determine the glucose content of a sample. its based on 2 coupled enzyme reactions with a colorimetric end-point:
D-glucose + O2 + H2O ----- glucose oxidase ------> H2O2 + gluconate
aminophenazone + phenol + H2O2 -----peroxidase----> a red dye + H2O
(NOTE: H2O2 product of 1st reaction acts as substrate for 2nd)
this red coloured sample can then be put into a spectrophotometer and an absorbency reading can be taken, this reading can be compared to a calibration curve and the content of glucose can be ascertained.
Wiki User
∙ 12y ago
Glucose oxidase is an enzyme that catalyzes the oxidation of glucose into gluconic acid and hydrogen peroxide. Peroxidase is another enzyme that uses hydrogen peroxide to catalyze the oxidation of various organic compounds. Aminophenazone is a compound that is commonly used in diagnostic tests to react with hydrogen peroxide in the presence of peroxidase, producing a colored compound. Phenol is an organic compound that can act as a reducing agent and is commonly used in various industrial applications.
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What happens to glucose in the phenol sulfuric acid test?
the glucose is hydrolised by the sulfuric acid. Furfural derivates are formed. These derivates form with phenol a yellow complex wich can be measured with a spectrophotometer.
Which is stronger acid ethanol or phenol?
phenol
Is phenol a non-electrolyte?
Phenol. Phenol's Dipole Moment: 1.7D Phenyl chloride's Dipole Moment: 1.54D
Can you get phenol from carbolic acid?
Carbolic acid is another name for Phenol.
What increase absorbance of phenol in ethanol?
Increasing the amount of phenol in the solution
What enzyme is present in liver and potatoes?
Catalase is the most well known enzyme common to both liver and potato, but there are others enzymes that can be found in potato. Oxidase and peroxidase are the other enzymes that can be found in potato. Oxidases in potato includes tyrosinase, cathecol oxidase and cytochrome oxidase. Peroxidase have the same function as with oxidase but they need a co-factor in order to complete its required action. The co-factor phenol oxidase is found abundant in potatoes. Hence, it will have a positive reaction once a substrate will be added indicating the presence of peroxidase in potatoes.
What are the products and reactants in an browning apple?
Browning of the apples is caused by the oxidation of the enzyme phenol oxidase:phenol oxidase + oxygen---------------melanins
What happens to glucose in the phenol sulfuric acid test?
the glucose is hydrolised by the sulfuric acid. Furfural derivates are formed. These derivates form with phenol a yellow complex wich can be measured with a spectrophotometer.
What is the function of polyphenol oxidase in plants?
Polyphenol oxidase helps in defense mechanism of plants. It converts toxic phenolic compounds into quinones which are not harmful to plants.
Poly phenol oxidase enzyme?
Translate text or webpage <span class="cs_misspelled">Polyphenol</span> <span class="cs_misspelled">oxidase</span> <span class="cs_misspelled">(tyrosinase)</span> Translate from: English Type text or a website address or translate a document. Cancel Example usage of "": automatically translated by Google Persian English Spanish Alpha Discovered the enzyme polyphenol oxidase (tyrosinase)
Why should peeled potatoes not to be exposed to air longer then necessary?
peeled potatoes should not be exposed to air longer than necessary to avoid oxidation and changing of color of the surface. it contains the polyphenol oxidase which oxidizes catechol to o-diphenol. the phenol oxidases are copper containing proteins which can oxidize phenol derivatives to quinones.
What is the reaction result for the methy red test for glucose on Staphylococcus epidermidis?
In my microbilogy lab, it was Glucose negative (meaning no gas or acid produced.) We used Phenol Red, however.
Structure of phenol?
phenol
Is phenol volatile?
Phenol is flammable.
Which is stronger acid ethanol or phenol?
phenol
What are some examples of a phenol?
What are some examples of Phenol
Why does temperature of water lowers on adding glucose?
It will increase the surface tension of water hence temperature decreases. If you add phenol to water temperature increase as it decreases the surface tension.
