How preper sudan3 solution?
Sudan III: To make 100 ml of Sudan IV staining solution: warm
73.5 ml of
95% ethyl alcohol [C2H5OH] in a warm water bath (hot tap water
will do).
Add 0.5 grams of Sudan (III) and stir. Add 75-80 degrees Celsius
distilled
water to just below the 100 ml mark; stir. Cool to room
temperature; dilute
to 100 ml with distilled water; stir then filter. (Use:
biological stain)