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The DNA polymerase enzyme synthesises the complementary DNA strand to a single stranded DNA strand (in vivo and in vitro). This often requires the presence of a 3' end for the polymerase enzyme to bind to before synthesis can begin. Taq polymerase (A DNA polymerase) is often used in PCR reactions to synthesise DNA in vitro using primers to provide a 3' end to bind to.
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How does DNA polymerase function in DNA replication?
DNA Polymerase III is responsible for adding new nucleotides to the strand being created. DNA Polymerase I replaces the primers with DNA nucleotides. The fragments are then joined together by ligase, and a new strand has been created.
What function does DNA polymerase have to enable it to detect errors and correct them?
proofreading
Principle enzyme involved in DNA replication?
DNA Polymerase.
What chemical is an enzyme that add nucleotides to a new strand of DNA during replication?
DNA Polymerase is the enzyme which adds new nucleotides during replication.
During DNA replication DNA is split apart into two strands as the new DNA strands are created what is the function of DNA polymerase?
The polymerase itself does not separate the DNA strands. Helicase (another enzyme, sometimes found in complex with a polymerase holoenzyme) does the separating for it, ahead of the replication fork.
What function does a DNA polymerase have?
To split DNA strands to create doubles.
What is the name of the two enzymes that help in the duplication of DNA?
More than two enzymes are involved. However, the main ones are DNA Polymerase I and DNA Polymerase III. DNA Polymerase III adds new nucleotides and DNA Polymerase I removes primers.
Which is the main enzyme used for the replication of DNA in ecoli?
DNA polymerase
What is the primase and its function?
Primase in the DNA-dependent RNA polymerase enzyme that functions in DNA replication by synthesizing the RNA primers which are then extended by DNA polymerase to yield newly synthesized DNA fragments. While being an RNA polymerase, primase is different from the RNA polymerase that functions in the transcription of DNA.
Which describes the main main purpose of RNA polymerase during transcription?
RNA polymerase produce mRNA from DNA
Two major enzymes used during DNA replication?
The two main ones are DNA-polymerase and helicase
How does DNA polymerase function in DNA replication?
DNA Polymerase III is responsible for adding new nucleotides to the strand being created. DNA Polymerase I replaces the primers with DNA nucleotides. The fragments are then joined together by ligase, and a new strand has been created.
What function does DNA polymerase have to enable it to detect errors and correct them?
proofreading
Principle enzyme involved in DNA replication?
DNA Polymerase.
What enzyme is used to join DNA nucleotides?
In DNA replication, DNA polymerase III is the enzyme which joins the DNA nucleotides together via phospodiester bonds.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.
What chemical is an enzyme that add nucleotides to a new strand of DNA during replication?
DNA Polymerase is the enzyme which adds new nucleotides during replication.
What is the function of a DNA?
There are different types of DNA polymerase depending if it's from a eukaryotic or prokaryotic cell each performing specific tasks. Basically DNA polymerase catalyzes the formation of a polymer, a DNA strand, from many monomers, deoxyribonucleotides.
