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Sumaira sultana

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Waldo Ledner

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2y ago

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What is the purpose of fixing the smear?

Heat fixing a smear kills the bacteria with minimal distortion, allows for better staining, and firmly affixes the bacteria to the slide. Chemical fixing is used to preserve fine cellular structures and might stop internal processes in place, protect the cell from damage, or strengthen the cell's structure.


Why must smear on the slide be completely dry before heat fixation?

Slight heating helps in fixing the cells on to the surface of the glass slide


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What are the precautions during preparation of bacterial smear and heat -fixing process?

During the preparation of a bacterial smear, it is crucial to use sterile techniques to avoid contamination. Use a sterile inoculating loop to transfer a small amount of culture onto a clean slide, and ensure the smear is thin and evenly spread. When heat-fixing, gently pass the slide through a flame to adhere the bacteria to the slide without burning them, as excessive heat can distort cellular structures. Always wear gloves and goggles to protect yourself from potential biohazards.


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What is meant by heat fixing a bacterial smear?

to allow the excess water to dry out before heating. if heated right after, the water would cause the smear to overheat and denature some features in the stain. and those features would no longer be seen


Why is it you should heat-fixing the air-dried before staining?

It is used to fix because to make the cell inactive or immoblie, but the main purpose is to fix the smear so that when we put stain and then flush it out with water ( or some time with alcohol) the smear should not wash out with dye.