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Why glucose and fructose form same osazone?
Glucose, Fructose and Mannose give the same osazone because these sugars have the same configuration of carbons 3, 4, 5 and 6. osazone formation only affects carbons 1 and 2.
Why glucose and fructose can be identified through osazone crystal formation?
They are both reducing sugars. They have aldose and ketose group at the side of the structure, which helps the sugar to condense with phenylhydrazine and produce solid derivatives called osazone. The solid is seen as crystals through the microscope.
What are two aldotetroses that yield the same osazone on reaction with phenyl hydrazine?
D-erythrose and D-threose both yield the same osazone. Likewise, L-erythrose and L-threose yield the same osazone.
Why osazone obtained from D-glucose and D-fructose have the same melting points?
glucose and fructose form same osazone crystals because carbon no 1 to carbon no 6 are identical both in glucose and in fructose
How many phenylhydrazines are required in osazone reaction?
3
What Reagents are used in osazone test?
Reagents of Osazone test are 1. Phenylhydrazine mixture consisting of phenyl-hydrazine and anhydrous sodium citrate 2. Orignal solution containing a carbohydrate
Why glucose and fructose form similar osazone crystals?
since carbons #3 through #6 of D-glucose and D-fructose molecules are identical, the sa,e osazone is formed.
Why acetic acid is mixed in the preparation of glucosazone?
osazone formation is unsatisfactory if it is not carried out in acidic medium. As minerel acid is formed and it hydrolyses the glucosasone. hence to carry out the reaction in acidic medium acetic acid is added and the mixture is made slight acidic by adding in -Abhishek Thakur
Does ribose form osazone crystal?
yes. pure galactose forms petal shaped crystals
Osazone test for carbohydrates?
osazone test is a carbohydrate specific reaction in which carbs react with phenylhydrazine to form osazones i.e. crystals of specific shape. thus the test confirms the presence of a particular sugar.Eg:for sucrose one gets a mixture of glucosazone and fructosazone on the basis of components of sucrose.
What is the importance of phenylhydrazine test?
The phenylhydrazine test is also known as the Osazone test. The phenylhydrazine test is used for reducing the amounts of carbohydrates, momosacchstides, and disaccharides.
What is the clinical significance of osazone test?
For identification purposes the carbonyl and adjacent alcohol functions will form phenylhydrazine derivatives known as osazones, which give characteristic melting points and exhibit definite crystalline structure. It should be noted that glucose , fructose and mannose yield the same osazone since the difference in structure and configuration about carbon atoms 1 and 2 are abolished.
