white peakcan see but dark peak can no see easily white peak can attaract eveerybody
Guru Shikhar is hill station in sirohi district of Rajasthan.It is the highest peak of rajasthan.It's height is 1722 m. After this peak SER peak is highest peak of Rajasthan.
They often do have a small peak in the center, but not always.
April, May, and June are generally the peak months of tornado activity in the U.S.
grossglockner
510 nm
Short answer:Using the maximum wavelength gives us the best results. This is because at the peak absorbance, the absobance strength of light will be at the highest and rate of change in absorbance with wavelength will be the smallest. Measurements made at the peak absorbance will have the smallest error.Long answer: It really depends on what is the largest source of error. Taking the readings at the peak maximum is best at low absorbance, because it gives the best signal-to-noise ratio, which improves the precision of measurement. If the dominant source of noise is photon noise, the precision of absorbance measurement is theoretically best when the absorbance is near 1.0. So if the peak absorbance is below 1.0, then using the peak wavelength is best, but if the peak absorbance is well above 1.0, you might be better off using another wavelength where the absorbance is closer to 1. Another issue is calibration curve non-linearity, which can result in curve-fitting errors. The non-linearity caused by polychromatic light is minimized if you take readings at either a peak maximum or a minimum, because the absorbance change with wavelength is the smallest at those wavelengths. On the other hand, using the maximum increases the calibration curve non-linearity caused by stray light. Very high absorbances cause two problems: the precision of measurement is poor because the transmitted intensity is so low, and the calibration curve linearity is poor due to stray light. The effect of stray light can be reduced by taking the readings at awavelength where the absorbance is lower or by using a non-linear calibration curve fitting technique. Finally, if spectral interferences are a problem, the best measurement wavelength may be the one that minimizes the relative contribution of spectral interferences (which may or may not be the peak maximum). In any case, don't forget: whatever wavelength you use, you have to use the exact same wavelength for all the standards and samples. See http://terpconnect.umd.edu/~toh/models/BeersLaw.htmlTom O'HaverProfessor Emeritus
When a protein in solution is analyzed using UV-visible, a peak at 280 nm is commonly observed. This peak is due to the effect of aromatic rings in the polypeptide chain (from amino acids tryptophan and tyrosine).
"absorbance"Since in the experiment, you probably choose the wavelength, then measure the absorbance (absorption?, the absorbance is the dependent variable.
Blank Sample in Spectrophotometry is used to measure the absorbance of light without sample. It is subtracted from the total absorbance for measurement of Absorbance from a sample's absorbance.
Blank samples are used to establish a baseline measurement of background contamination in an analysis. By analyzing a blank sample containing no target analytes, researchers can identify and account for any background signals or contamination that may affect the accuracy of their results.
specific absorbance- it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell. so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light. divya.chakraborty@gmail.com
in primary light absorbed by outer molecule while in secondary re-absorbance occurs
Because when we read absorbance, it's the amount of light absorbed by the bacteria itself. Absorbance is directly related to the amount of bacteria. More absorbance = more bacteria.
If you have a spectrofotometer ( the thing to mesure the absorbance) then play with the setting and use a maximum. this will lay close to your specific absorbance or take the pharmacopea or a MERCK index
A
because that chart gives a more accurate value than the absorbance scale on the specthometor