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What else can I help you with?
What is difference between specific absorbance and absorbance interms of spectroscopy?
specific absorbance- it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell. so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light. divya.chakraborty@gmail.com
Is absorbance considered a discrete or continuous variable?
Absorbance is considered a continuous variable.
Does acetone reacts with acetone?
No, because when you add acetone to acetone, all you are doing is adding more of the volume of acetone to acetone. You are just changing the amount of acetone, not anything chemically happening.
What is the name of CH3COCH3?
Its a polyatomic ion called Acetate
Is acetone vapor or gas?
Acetone exists as a liquid at room temperature but can evaporate to form acetone vapor, which is a gas made up of acetone molecules.
Why does protein have an absorbance peak at 280nm?
When a protein in solution is analyzed using UV-visible, a peak at 280 nm is commonly observed. This peak is due to the effect of aromatic rings in the polypeptide chain (from amino acids tryptophan and tyrosine).
What is the principle behind Quantification of DNA by reading its optical density at 260nm and 280nm?
The principle behind quantifying DNA by measuring its optical density at 260nm and 280nm is based on the fact that DNA absorbs light at these specific wavelengths. The ratio of the absorbance at 260nm to 280nm is used to assess the purity of the DNA sample, with a 260/280 ratio of around 1.8 considered indicative of pure DNA. By comparing the absorbance values at these two wavelengths, scientists can estimate the concentration and purity of DNA in a sample.
How do you find whether given sample is DNA or RNA?
A spectrophotometer can be used to know if a sample is DNA or RNA. DNA has an absorbance maximaat 260nm, whereas RNA has an absorbance maxima at 280nm. By looking at which one of these two wavelengths the sample is more excited, one can determine if the sample is DNA or RNA.
A diluted protein solution (1 in 100) has an absorbance of 0.558 at 280nm when measured in a 1cm path-length cuvette. Calculate the concentration in mgmL of the undiluted protein solutions (extinctio?
One needs the extinction coefficient in order to answer this question. Otherwise it cannot be answered properly.
What is the torque settings for Renault captur wheel nut?
280Nm
What is the specific absorbance of hypericin?
Hypericin salts are red in organic solvents and show a typical absorbance at 590 nm, which is useful to quantify hypericin in the drug extracts
Is wavelength or absorbance the dependent variable?
"absorbance"Since in the experiment, you probably choose the wavelength, then measure the absorbance (absorption?, the absorbance is the dependent variable.
What is the purpose of the blank?
Blank Sample in Spectrophotometry is used to measure the absorbance of light without sample. It is subtracted from the total absorbance for measurement of Absorbance from a sample's absorbance.
What is difference between specific absorbance and absorbance interms of spectroscopy?
specific absorbance- it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell. so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light. divya.chakraborty@gmail.com
Is absorbance considered a discrete or continuous variable?
Absorbance is considered a continuous variable.
What is the different between primary absorbance and secondary absorbance in fluorescence?
in primary light absorbed by outer molecule while in secondary re-absorbance occurs
What is the specific absorbance of aspirin?
If you have a spectrofotometer ( the thing to mesure the absorbance) then play with the setting and use a maximum. this will lay close to your specific absorbance or take the pharmacopea or a MERCK index
