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in primary light absorbed by outer molecule while in secondary re-absorbance occurs

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What is the difference between spectrophotometer and fluorimeter?

A spectrometer shoots light through a sample and detects absorbance while a fluorimeter detects the intensity of fluorescence of a given sample.


Can you explain the relationship between absorbance and wavelength in a graph?

In a graph, absorbance is typically shown on the y-axis and wavelength on the x-axis. The relationship between absorbance and wavelength is that as the wavelength of light increases, the absorbance generally decreases. This is because different substances absorb light at specific wavelengths, so the absorbance of a substance can vary depending on the wavelength of light being used.


What is the relationship between the wavelength of light and absorbance in a given substance?

The relationship between the wavelength of light and absorbance in a substance is that different substances absorb light at specific wavelengths. This absorption is measured as absorbance, which increases as the substance absorbs more light at its specific wavelength.


What is the relationship between wavelength and absorbance in a spectrophotometry experiment?

In a spectrophotometry experiment, there is an inverse relationship between wavelength and absorbance. This means that as the wavelength of light increases, the absorbance decreases, and vice versa.


What is difference between an HPLC UV detector and a fluorescence detector?

HPLC UV detectors measure absorbance of UV light at a specific wavelength, while fluorescence detectors measure the emission of light at a longer wavelength after excitation with UV light. Fluorescence detectors are more sensitive and selective than UV detectors, but may require additional steps such as derivatization for certain compounds.


What is the relationship between absorbance and wavelength in spectroscopy?

Absorbance in spectroscopy is directly related to the wavelength of light being used. As the wavelength increases, the absorbance typically decreases. This relationship is important for determining the concentration of a substance in a sample based on the amount of light it absorbs at different wavelengths.


How does the relationship between wavelength and absorbance impact the absorption spectrum of a substance?

The relationship between wavelength and absorbance affects the absorption spectrum of a substance because different substances absorb light at specific wavelengths. As the wavelength of light changes, the absorbance of the substance also changes, resulting in a unique absorption spectrum that can be used to identify the substance.


What is the relationship between the absorbance of tryptophan and its concentration in a given solution?

The relationship between the absorbance of tryptophan and its concentration in a solution is direct and proportional. As the concentration of tryptophan in the solution increases, the absorbance of light by the solution also increases. This relationship is described by the Beer-Lambert Law, which states that absorbance is directly proportional to concentration.


What is the relationship between the optical density (OD) and absorbance in spectrophotometry?

In spectrophotometry, optical density (OD) and absorbance are directly related. As the OD increases, the absorbance also increases. This means that a higher OD value indicates a higher absorbance of light by the sample being measured.


What is difference between specific absorbance and absorbance interms of spectroscopy?

specific absorbance- it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell. so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light. divya.chakraborty@gmail.com


What is the relationship between absorbance and transmittance?

Absorbance = -log (percent transmittance/100)


Why is the absorbance versus concentration curve rarely straight?

The absorbance versus concentration curve is rarely straight because the relationship between absorbance and concentration is not always linear. Factors such as non-linear interactions between molecules, chemical reactions, and instrumental limitations can cause the curve to be curved instead of straight.