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Why the retention time will decrease if the polarity of mobile phase increase in normal phase HPLC?
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
Which type of samples analysed by hplc?
The samples that are not volatile and are soluble in the mobile phase.
What is the H.P.L.C. chromatography?
HPLC stands for high performance liquid chromatography. It is a liquid chromatography which involves the separation of the compounds on the basis of their polarity. It is used to analyze, identify, purify & quantify the compounds.
Can C18 column for HPLC be used with UV and visible spectra detector and Acetonitril as mobile phase?
Of course.... there isn´t problem....
How does hplc works?
HPLC works when a reservoir holds the solvent and then it is sent to the pump manager.Next it goes to the HPLC coloumn .After it goes through there it usually ends in the detector than waste. Generally the stationary phase in the HPLC column is made up of alkyl coated silica making it relatively non-polar. Due to this the technique is also called reversed-phase HPLC.
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
What is an HPLC column?
HPLC Column is one type of tube containing a stationary phase react with mobile phase to detect peak
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Why the retention time will decrease if the polarity of mobile phase increase in normal phase HPLC?
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
Which type of samples analysed by hplc?
The samples that are not volatile and are soluble in the mobile phase.
How you can analyze pregabalin on HPLC with UV detector?
We can quantitatively analyse pregabalin on hplc with uv detector, wavelength will be 210 n.m. and mobile phase will be 5 % acetonitrile. standard & sample solution preparation should be in mobile phase.
How HPLC is used as separation technique?
it used a mobile phase to carry a sample through a tube containing a stationary phase which is a liquid coated on solid particles.
What is done to revers direction of a three phase electric motor?
To reverse direction, swap any two hot wires.
What is the H.P.L.C. chromatography?
HPLC stands for high performance liquid chromatography. It is a liquid chromatography which involves the separation of the compounds on the basis of their polarity. It is used to analyze, identify, purify & quantify the compounds.
What is dead volum in hplc?
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
Can C18 column for HPLC be used with UV and visible spectra detector and Acetonitril as mobile phase?
Of course.... there isn´t problem....
How does hplc works?
HPLC works when a reservoir holds the solvent and then it is sent to the pump manager.Next it goes to the HPLC coloumn .After it goes through there it usually ends in the detector than waste. Generally the stationary phase in the HPLC column is made up of alkyl coated silica making it relatively non-polar. Due to this the technique is also called reversed-phase HPLC.
