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We can quantitatively analyse pregabalin on hplc with uv detector, wavelength will be 210 n.m. and mobile phase will be 5 % acetonitrile. standard & sample solution preparation should be in mobile phase.
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What is Difference Between HPLC UV detector Spectrophotometer uv detector?
hi, could any one answer what is the difference between hplc uv detector and spectrophotometer uv detector ? my id is debendra_mallick2002@yahoomail.com
What is difference between an HPLC UV detector and a fluorescence detector?
flourescence is more sensetive than UV detection
Why is Erbium perchlorate is used in calibration of HPLC?
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
Can C18 column for HPLC be used with UV and visible spectra detector and Acetonitril as mobile phase?
Of course.... there isn´t problem....
How Lambda max determined for quetiapine fumarate?
by injecting the sample in to a HPLC system having PDA detector or Scan the Quetiapine Fumarate sample solution in UV range.
What difference between pda and uv detector in hplc?
PDA - Photo diode array UV- Ultra violet with use of PDA detector, we can measure the area or height of particular peak at different wavelengths ranging from 200 to 800 nm by injecting the solution at once. Where as in uv detector we can measure the area or height of the peak only at two different wavelengths. But that wavelengths also to be selected before injecting the solution.
What is the lambda max of pregabalin?
uv max of pregabalin are following: 1.in water 276nm 2.in methanol 210nm
What is the function of detector in UV spectrophotometer?
Detector converts optical signal to electric signal
Why acetonitrile is not detected in uv dector in hplc?
COZ ACN ABSORVE LESS THEN 220nm.
What is different between pda detecto and uv detector?
technical difference is flow cell is placed in between lamp and optics but in uv it is placed after grating. and flow cell receives monochromatic light in uv detector but polychromatic in pda.
Why to analyze formaldehyde by using 412 nm in the Uv-Vis?
it is the absorption maximum for formaldehyde...
What are the columns used in hplc?
* large columns, specifically for packing. * analytical columns, for quantitative analysis, usually accompanied by a UV-vis detector. * Narrow bore columns, for more sensitive analysis * capillary columns, very this silica columns used almost exclusively with GC mass spectroscopy. * packed bed columns. with silica beads. and may have groups attached, e.g. C18.
