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Where is the dna placed in gel electrophoesis apparatus?
In the well made in gel with gel loading buffer
What is a difference between 2 percent and 3 percent agarose gel?
the thickness of the gel varies as you change the conc. of agarose from 2 to 3 percent. the conc. of loading of samples that gel can handle varies.
Why gel loading dye give different colouration with DNA sample?
This can happen because of the pH variation in your dna sample or the chloroform:isoamyl alcohol was not properly removed during the washing step.
What is an example of a gel?
example of gel is agarose gel,
While loading silica gel in column for column purification large scale why does outer surface of the column become warm?
Assuming it isn't waste heat from the surroundings, friction is the likely source, either with the sides of the containing vessels or from the pressure of compaction.
Where is the dna placed in gel electrophoesis apparatus?
In the well made in gel with gel loading buffer
What is a difference between 2 percent and 3 percent agarose gel?
the thickness of the gel varies as you change the conc. of agarose from 2 to 3 percent. the conc. of loading of samples that gel can handle varies.
What is staking in crop production?
Staking of plants which have climbing or straggling habit of growth.
How do you spell staking-out?
The literal term "staking out" an animal or "staking out" (marking) a claim are just separate words. The police term meaning surveillance is a "stakeout."
What is the least loading volume for western blot?
Least loading can be about 2-5 microliters. It is normally depends on the size of the well you make on the gel. And it doesnt matter you add how much because how much concentrated is crucial for loading. adding 2ul of 10X sample is equal to loading 20ul of 1X sample.
What is used to make the DNA visible on the gel?
YES!! You can use a simple Agarose gel to separate to view the DNA on electrophoresis. Use 0.8 - 1% gel for 5-10kbp , 2% for 0.2 - 1kbp. If the fragments are really tiny, use an Acrylamide gel (vertical gel) to electrophorese and they will show right out. This is to offset the instability of high concentration gels.
Is Google staking me?
yes they are
What actors and actresses appeared in Pain Staking - 2012?
The cast of Pain Staking - 2012 includes: Christopher Neiman as Phil
What jobs did Children have on the Oregon Trail?
gathering fire wood, staking the horses unloading the wagon and loading it the next morning, help adults, looked for dried buffolo manure
What actors and actresses appeared in Staking His Life - 1918?
The cast of Staking His Life - 1918 includes: Louise Glaum as Bubbles Charles Ray as Frank Hamilton
What is the Composition of gel loading dye used in DNA amplification?
Sucrose and Bromophenol blue (6X): 4gm sucrose 25mg bromophenol blue (0.25%) Distilled water to 10 ml
Why gel loading dye give different colouration with DNA sample?
This can happen because of the pH variation in your dna sample or the chloroform:isoamyl alcohol was not properly removed during the washing step.
