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Supercritical fluid chromatography is very similar to high pressure liquid chromatography (HPLC), however the mobile phase exists in a supercritical state. To refresh your memory, recall that super critical fluids exist at temperatures and pressures higher than a substances critical point. This means that the fluid acts as both a gas and a liquid. For example, a supercritical fluid may pass through substances like a gas, but also dissolve substances like a liquid.

Why do supercritical fluids exist? Well, the temperature is high enough that the molecules cannot exist together as a fluid because they have too much energy to stick together. However, the pressure is too high for the molecules to have a root mean square distance sufficient enough to exist as a gas.

SFC exploits this property, using a supercritical fluid to dissolve the chemical in question into the fluid, but move it quickly in the gas phase. Heavy molecules travel slower, while light molecules travel faster, thus causing separation. In addition, chiral molecules can be separated via this method.

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Q: What is supercritical fluid chromatography?
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