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Why use iodine when preparing slide for viewing?
one disadvantage of using iodine to stain cells is that iodine will kill living cells.
A what treatment is the oral administration of radioactive iodine to destroy thyroid cells?
Addison's disease is treated using the oral administration of radioactive iodine to destroy thyroid cells.
What is advantage of using stain?
Using stains on slides help show some details that may be unclear otherwise, especially in cells.
What happens if grams iodine is not applied after staining with crystal violet?
If the iodine in the Gram's stain was omitted, the crystal violet which enters the cell will not be able to form the Crystal Violet Iodine (CVI) complex. The CVI complex molecule has a larger size than that of Crystal violet molecule and hence Gram +ve bacteria are able to retain the stain. If iodine were omitted, even Gram+ve cells would get decolorised by Alcohol treatment and would be seen as Gram-ve cells.
How does iodine stain starch?
Starch is composed of polymers of glucose. Long linear chains are called amylose. Amylopectin is similar but contains a branch point about every 25th glucose or so.Amylose coils into a helical secondary structure resembling a tube with a hollow core. Certain molecules including fatty acids and iodine can lodge inside the core. The complex of iodine lodged inside the amylose tube produces a characteristic blue-black color. The starch itself is not altered, and the staining can be reversed.This is a very sensitive method, used industrially in the starch processing industry, to detect starch and starch fragments. Levels down to 10 ppm can be quantitated using a spectrophotometer to measure A620.However, Iodine is a "metachromatic" stain, meaning it is a single stain that can stain different things different colors. For example: Although Amylose in corn starch stains bluish (actually a sort of blue with a reddish-purple tent), Amylopectin type starches stain a completely different color, sort of a reddish-brown. Tapioca starches stain a more true blue color. Additionally, high amylose type starches stain a shade of pink (when viewed using a polarized microscope, with cross-polarizers in place). Therefore, it is a mistake to think that "all starches" stain a bluish color.Additionally, varying the concentration of the stain, relative to a given amount of starch, can vary the intensity of the staining result. Less stain produces a lighter shade of the expected color, and vice-versa. And the best Iodine stain is actually a blend of Iodine crystals and Potassium Iodide in water.CheapIodine.com is a great source for Iodine.
Function of acetone in gram staining?
Acetone is used for decolouring method, washing away the iodine-crystal violet complex formed in gram negative bacteria. so that the gram negative bacteria can be then stained with safranin or fuchsin.
Can a Gram stain a prion?
Protists are often stained using a silver stain, not a Gram stain.
What is iodine used to see when using a microscope?
Iodine can be used to identify starch.
If iodine step missed in gram stain what will happen?
Iodine (I - or I3 - ) interacts with CV+ and forms large complexes of crystal violet and iodine (CV-I) within the inner and outer layers of the cell. Iodine is often referred to as a mordant, but is a trapping agent that prevents the removal of the CV-I complex and, therefore, color the cell.[10]
When using iodine on a potato or corn the iodine turns black What is this evidence of?
Presence of starch.
What are the nutritional benefits of iodine?
Iodine is very useful for our body. Iodine deficiency can cause thyroid. So just check your iodine level in your salt which you are using in your food. Better use Iodine Salt.
Why does iodine work when using to find fingerprints?
probably because iodine sticks to the oil from your fingers
