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What is the chemistry of the CHOD-PAP method of cholesterol measurement?
Wiki User
∙ 11y ago
Chemistry of the CHOD-PAP method of cholesterol measurement
The cholesterol is determined after enzymatic hydrolysis and oxidation.
The indicator quinoneimine is formed from hydrogen peroxide and
4-aminoantipyrine in the presence of phenol and peroxidase.
Cholesterol
Cholesterol ester + H2O ----------------------> Cholesterol + Fatty acids
esterase
Cholesterol
Cholesterol + O2 ----------------------------->Cholestene-3-one + H2O2
oxidase
Peroxidase
2H2O2 + phenol + 4-Aminoantipyrine ---------------------------> quinoneimine + 4H2O
Wiki User
∙ 11y ago
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