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Answered 2013-03-13 21:22:44

Chemistry of the CHOD-PAP method of cholesterol measurement

The cholesterol is determined after enzymatic hydrolysis and oxidation.

The indicator quinoneimine is formed from hydrogen peroxide and

4-aminoantipyrine in the presence of phenol and peroxidase.

Cholesterol

Cholesterol ester + H2O ----------------------> Cholesterol + Fatty acids

esterase

Cholesterol

Cholesterol + O2 ----------------------------->Cholestene-3-one + H2O2

oxidase

Peroxidase

2H2O2 + phenol + 4-Aminoantipyrine ---------------------------> quinoneimine + 4H2O

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