Exercise
Chemistry

What is the chemistry of the CHOD-PAP method of cholesterol measurement?

User Avatar
Wiki User
2013-03-13 21:22:44

Chemistry of the CHOD-PAP method of

href="http://cholesterol.answers.com" title=

"cholesterol">cholesterol measurement

The cholesterol is determined after enzymatic hydrolysis and

oxidation.

The indicator quinoneimine is formed from hydrogen peroxide

and

4-aminoantipyrine in the presence of phenol and peroxidase.

Cholesterol

Cholesterol ester + H2O ----------------------> Cholesterol +

Fatty acids

esterase

Cholesterol

Cholesterol + O2

----------------------------->Cholestene-3-one + H2O2

oxidase

Peroxidase

2H2O2 + phenol + 4-Aminoantipyrine

---------------------------> quinoneimine + 4H2O


Copyright © 2020 Multiply Media, LLC. All Rights Reserved. The material on this site can not be reproduced, distributed, transmitted, cached or otherwise used, except with prior written permission of Multiply.