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There is absolutely no way to tell the difference. When the frogs reach sexual maturity, the males will call for a mate and the females do not. That's about the only reliable way to tell with most species. A distress call is not a mating call, so holding the frog tightly is not an accurate way to determine the frog's sex. You just have to wait.
(The difference is, one's a boy & one's a girl-(sorry, couldn't resist this answer:)
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