Want this question answered?
Denature enzyme activity
Cold temperatures have a drastic effect on an enzyme's activity level. Cold temperatures usually dramatically slow down an enzyme's activity.
pH
The pH is varied to effect, by its affect, this test.
temperature and pH
At low concentration of substrate , rate of enzyme action is directly proportional to conc. of substrate .
many vitamins and minerals play crucial roles in many metabolisms as coenzyme or cofactor. Deficiency of those lower the related-enzyme activity.
The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity
Physical activity can alter the shape of enzyme which can cause damage or may the enzyme become inactive
It would lower the speed of the reaction but will do no harm to the enzyme.
effect of ph and concentration of subtrate
Different enzymes work best at different pH. This is refered to as the ideal pH for the enzyme. For example, the digestive enzyme trypsin works best at an acidic pH while alkaline phosphatase works best at a basic pH. Therefore, enzyme activity varies with pH and this variation depends on the enzyme being studied