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DNA Polymerase
Unwinds the DNA during DNA replication and Trancription
DNA helicase is the enzyme that aids DNA in unzipping during transcription.
At the hydrogen bonds between bases with the help of the enzyme helicase.
A Retsriction enzyme endonuclease is an enzyme that is used to cut DNA strands (both single and double strands) during finger printing at the DNA recognition sites known as restriction sites.
DNA Polymerase
polymerase
Unwinds the DNA during DNA replication and Trancription
DNA helicase is the enzyme that aids DNA in unzipping during transcription.
At high temperatures, DNA denatures into single strands. A temperature around 95 degrees should work.
single-strand binding proteins
At the hydrogen bonds between bases with the help of the enzyme helicase.
A Retsriction enzyme endonuclease is an enzyme that is used to cut DNA strands (both single and double strands) during finger printing at the DNA recognition sites known as restriction sites.
ligase
DNA Helicase is the major enzyme involved in the replication of DNA. The reason why it is so important is that it unwinds the DNA which creates two separate strands.
Separation of the two DNA strands by the action of the Helicase enzyme.
During DNA replication the following occurs: 1) An enzyme called helicase separates the DNA strands (the space where they separate is called the replication fork). 2) DNA polymerase adds complementary nucleotides to the separated strand of DNA. 3) The DNA polymerase enzyme finishes adding nucleotides and there are two identical DNA molecules.