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Bonds to the phosphate
preparation of 5.8 ph phosphate buffer
By dilution of 1 part 0.50M buffer with 49 parts of water, giving 50 parts of the desired 0.010M = 10 mM Phosphate buffer
5 mM phosphate buffer (4.82 g/l monohydrate, monosodium phosphate, pH 6.5).
The buffer system that operates in blood plasma is the bicarbonate buffering system. The chemical equation for this system is the following CO2 + H2O <--> H2CO3 <--> HCO3- + H+.
1. Why is the phosphate buffer made up by using the Henderson-Hasselbalch equation not the expected pH?
1. Bicarbonate buffer system 2. Protein buffer system 3. Phosphate buffer system
bicarbonate buffer is instant, followed by respiratory, renal, and phosphate.
Bonds to the phosphate
Yes you need to know what molarity of the phosphate buffer you need to make and what voluime, then you can use Henderson-Hasselbalch equation. or simply use the phophate buffer calculator http://home.fuse.net/clymer/buffers/phos2.html
its ok to use
preparation of 5.8 ph phosphate buffer
By dilution of 1 part 0.50M buffer with 49 parts of water, giving 50 parts of the desired 0.010M = 10 mM Phosphate buffer
buffer systems function mainly to regulate the acid or base balance in the body. there are 3 principal classes of buffers in the body - proteins, phosphate buffer system and the bicarbonate buffer system. however, you are asking of the organs involved. these are the kidneys and the lungs.
Bicarbonate Buffer System (only important ECF buffer)
5 mM phosphate buffer (4.82 g/l monohydrate, monosodium phosphate, pH 6.5).
The blood contains buffers like bicarbonate and phosphate buffers. The bicarbonate buffer is by far, the most important.