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EDTA is a chelating agent and has great affinity with matel ions and Mg-ion present in DNase as a cofactor and responsible for DNase action that degrade the DNA,here EDTA bind with Mg-ion and nullyfy the action of DNase.

The nuclear envelope normally protects the DNA from digestion by nucleases. Nuclear envelope is the membrane that surrounds the nucleus and prevents the exposure of its contents such as the DNA to the contents of cytoplasm. In the process of DNA extraction, we need to break down the nuclear envelope in order to access the DNA. This would expose the DNA to nucleases and if we don't deactivate these enzymes, they will cut and damage the DNA. Nucleases need divalent cations such as Mg2+ to function. In order to deactivate these enzymes we use EDTA which stands for Ethylenediaminetetraacetic acid to our sample tissue. EDTA has four carboxyl groups ( -COOH). In the alkaline condition of the buffer, EDTA becomes negatively charged. The EDTA ions then form covalent bonds with the divalent cations and prevent them from reacting with nucleases. As a result, the enzymes are deactivated and will no longer cause a threat to the DNA.

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โˆ™ 2012-08-09 18:11:09
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Q: What is the function of EDTA in DNA extraction?
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Role of saline tris edta in DNA extraction?

Its purpose is to isolate DNA from a protein mixture.


What is the function of Tris cl in extraction of DNA?

it interacts with the lipopolysaccharides found in the outer membrane. by this it is helping the work of EDTA. this will cause cell lysis


What is function of tris BUFFER in DNA extraction?

Buffer is used to maintain pH in any process. In DNA extraction EDTA used as a chelating agent at pH8. This ensure the isolation process pure, since chelating agent inactivate the contaminating DNAses.


What is the function of tris and EDTA buffer?

solubilize DNA


What is content se- buffer in DNA extraction?

Tris pH 8.0 NaCl EDTA


Role of te in dna extraction?

TE stands for Tris and EDTA. The Tris buffers the water to prevent acid hydrolysis of the DNA/RNA. The EDTA chelates divalent cations that can assist in the degradation of RNA.


What is the function of TRIS in DNA extraction?

TRIS maintains the pH of the solution. Basically it interacts with the lipopolysaccharides present on the outer membrane which helps to permeabilize the membrane. This effect is enhanced with the addition of EDTA.


What is the function of TBE in extraction of DNA?

TBE (Tris Borate EDTA).. TBE usually use like TAE in electrophoresis.. The difference is the size of molecule DNA that you want to separate. You can use TBE for the small size than TAE.


What is the function of BL buffer in DNA extraction?

In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.


What is the function of lysozyme in DNA extraction?

Lysozyme is an enzyme that is used during DNA extraction to degrade the cell wall.


What is the function of cold water in dna extraction?

Cold water helps keep the DNA intact during extraction.


What the function of EDTA during DNA extraction?

EDTA has high affinity towards divalent ions like Ca2+, Mn2+, Mg2+ which are cofactors for many active enzymes inside the cells. That includes nucleases which digests DNA molecules. Once the cell is disrupted, nuclear envelope goes off and the nuclear content comes into contact with the cellular content which is rich in nucleases. So the broken cell is treated with EDTA to chelate the ions so that nucleases loose their function and that we are able to get good yield of DNA.


Function TE in extraction DNA?

chelating Mg2+


What is the function of Buffer BL in a DNA extraction?

In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.


What is the function of chloroform in DNA extraction?

It solubalize lipids and protiens to remove them from DNA


What is the function of dithiothreitol in DNA extraction?

DTT is the reducing agent for thiolated DNA


What is the function of 2-propanol in DNA extraction?

To achieve precipitation DNA.


What is function of Nacl in DNA extraction?

Sodium chloride help the the precipitation of DNA.


What is the function of EDTA during protein extraction?

glycerol increases the stabilization of the protein by decreasing the surface tension of water


Function of NACL in DNA extraction?

maintain ions in buffer....


What is the function of ascorbic acid in DNA extraction?

removal of polyphenols


What the function of SDS during DNA extraction?

It lyses the cell membrane and nucleus to make the extraction possible


Function chloroform in extraction DNA from blood?

to remove excess phenol from DNA to remove excess phenol from DNA


What is the function of NaCl in DNA extraction?

Sodium chloride help to separate DNA from other proteins.


What is the function of ammonium acetate in DNA extraction?

ammonium acetae use to percipitate DNA from water.