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EDTA has high affinity towards divalent ions like Ca2+, Mn2+, Mg2+ which are cofactors for many active enzymes inside the cells. That includes nucleases which digests DNA molecules. Once the cell is disrupted, nuclear envelope goes off and the nuclear content comes into contact with the cellular content which is rich in nucleases. So the broken cell is treated with EDTA to chelate the ions so that nucleases loose their function and that we are able to get good yield of DNA.
What else can I help you with?
Write down the reaction equation of Zn2 plus and EDTA?
The reaction equation between Zn^2+ and EDTA is: Zn^2+ + EDTA → Zn(EDTA)^2-
What is tertrasodium edta?
Tertrasodium EDTA is a chelating and preservative agent.
How many grams of EDTA do you add to make a 3.7 percent EDTA solution?
To make a 3.7% EDTA solution, you would add 3.7 grams of EDTA to 100 mL of solution.
What is colour of EDTA metal complex?
what is colour of Mg2plus- EDTA complex?
When edta is insoluble in distilled water then how to make 0.01N edta solution?
use heat to heat the solution and add EDTA slowly to dissolve it.
Why ph8 is maintained during isolation of plasmid?
EDTA is dissolved only at pH8. EDTA serves as an important chelating agent to kill the contaminating DNAses. Also this is close to the physoological pH which is pH7.
What is the Role of RNAase in plasmid preparation?
RNAase is used in plasmid preparation to degrade RNA contaminants present in the sample. This helps to ensure that the isolated plasmid DNA is free from RNA, which can interfere with downstream applications such as PCR or cloning. RNAase treatment is an important step to obtain high-quality plasmid DNA.
Why ammonia is added in preparation of EDTA solution?
Ammonia is added in the preparation of EDTA solution to create a slightly basic pH environment, which helps to maximize the stability of EDTA and improve its chelating ability. The ammonia also helps to prevent the precipitation of certain metal hydroxides that may interfere with the chelation process.
Why is Ammonia solution added while preparing EDTA solution?
Ammonia solution is added to increase the pH of the solution to create a favorable environment for the formation of stable metal-EDTA complexes. This helps in improving the efficiency of complexation and enhances the chelating properties of EDTA.
You have propagated a vector in a non expression host and isolated the plasmid How can you confirm whether the plasmid that you got is exactly the vector which you propagated?
Check the size in agarose gel, extract it from the gel then purify it and grow it on selective plate.
Which is the plasmid that increases resistance to antibiotics?
R-plasmid
What plasmid that can degrade toluic acid?
TOL plasmid
Write down the reaction equation of Zn2 plus and EDTA?
The reaction equation between Zn^2+ and EDTA is: Zn^2+ + EDTA → Zn(EDTA)^2-
What is tertrasodium edta?
Tertrasodium EDTA is a chelating and preservative agent.
How many grams of EDTA do you add to make a 3.7 percent EDTA solution?
To make a 3.7% EDTA solution, you would add 3.7 grams of EDTA to 100 mL of solution.
How can i know if my bacteria contain plasmid or not?
You can determine if your bacteria contain a plasmid by performing a plasmid extraction followed by gel electrophoresis to visualize the presence of plasmid DNA. Other methods include PCR amplification of plasmid-specific sequences or using molecular biology techniques like restriction enzyme digestion to confirm the presence of a plasmid.
What is colour of EDTA metal complex?
what is colour of Mg2plus- EDTA complex?
