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What type of medium is motility medium?
Motility medium is a type of semisolid medium used in microbiology to assess the motility of microorganisms. It typically contains a lower percentage of agar compared to solid media, allowing motile bacteria to move through the medium and produce observable growth patterns.
Why is the pH of the medium important in edta titration?
The pH of the medium is important in EDTA titration because the formation of the metal-EDTA complex depends on the pH. At certain pH levels, the metal-EDTA complex formation is optimized, leading to accurate results. Deviations from the optimal pH can affect the stability of the complex and lead to incorrect titration results.
Why do you use Fe-EDTA in murashige and skoog medium?
Fe-EDTA is used in Murashige and Skoog medium as a source of iron for plant tissue culture. Iron is an essential micronutrient required for proper plant growth and development. Fe-EDTA provides a stable form of iron that is readily available to plants in the medium.
If sim medium was used for motility determination for Proteus vulgaris what noticeable change to the medium will be observed?
A noticeable change in the medium due to Proteus vulgaris motility would be the presence of diffuse growth radiating from the point of inoculation, resulting in a fuzzy or feathery appearance on the surface of the agar. This motility pattern is characteristic of Proteus species, which exhibit swarming motility on semisolid media like SIM medium.
Why is the titration not carried in acid medium in edta method?
Titration is not carried out in an acid medium in EDTA method because at low pH levels, the formation of metal-EDTA complexes is hindered, leading to inaccurate results. The EDTA molecule itself is stable at a slightly alkaline pH, which enhances its chelating ability and ensures more accurate complex formation with metal ions.
Why is motility medium inoculated only one half the way down the tube?
Motility medium is inoculated only halfway down the tube to observe the migration of motile bacteria from the point of inoculation. This allows for the determination of the motility of the bacteria based on their ability to move away from the point of inoculation. If the bacteria are motile, they will spread throughout the medium, resulting in turbidity along the line of inoculation.
Why is motility medium semi-solid?
Because it does not inhibit bacteria form "swimming" through the medium.
What are the methods used for identifying motility?
There are two basic method to determine motility in a bacterial sample. These samples have similar biochemical identifiers. The first test is a simple drop test. The second method employs a motility medium
What effect does EDTA have when added to a lactase meditated reaction?
EDTA removes the ions that lactase needs to function as an enzyme. If enough EDTA is added, lactase will no longer have any of it's ion cofactors to aid in the break down of lactose.
How is SIM medium used to detect motility?
SIM agar may be used to detect motile organisms. Motility is recognized when culture growth (turbidity) of flagellated organisms is not restricted to the line of inoculation. Growth of non-motile organisms is confined to the line of inoculation.
What is the function of EDTA in DNA extraction processes?
EDTA is used in DNA extraction processes to chelate divalent cations, such as magnesium, which are necessary for the activity of DNases that can degrade DNA. By removing these cations, EDTA helps protect the DNA from degradation during the extraction process.
What factors dictate the choice of tests included in a combination medium?
The hydrogen sulfide production, motility, and indole production.
