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Hi I am going to make note of the source for the sake of convineance: Conoley C, (2002), Chemistry, London: HarperCollinsPublishers ISBN: 0-00-713597-1 (page no:418).
Positiveley charged ions migrate to the cathode (-ve) and negativeley charged ions to the anode (+ve). When the pH is that at which zwitterions* are fromed (the isoelectric point) with equal positive and negative charges the zwitter ions cannot move in either direction. so, the pH value must be carefully chosen before the mixture of amino acids can be separated xan be separated and analysed successfully.
*A zwitterion is formed when an amino acid undergoes internal neutrilisation, by this I mean the the H+ ion on the COOH group goes to the NH2 group (the amine group) to form an NH3 group thus for one is positive (NH3+) and one is negative (COO-) and they are therefore neutral.
NB: Gel electrophoresis works on the separation of proteins (and therefore DNA aswell) don't forget that amino acids are what make up proteins so don't at any point think that the reference to amino aicds in this context is abstract.
Hope this helps Nick C
What else can I help you with?
What is the function of a buffer in gel electrophoresis?
A buffer in gel electrophoresis helps maintain a stable pH level and provides ions for conducting electricity, allowing the DNA or proteins to move through the gel.
What is the function of buffer in the gel electrophoresis?
TBE buffer in gel electrophoresis is used to maintain pH of te solution and prevents the denaturation of smale fragments of DNA.
What is a common name for gel electrophoresis?
Agarose gel electrophoresis.
Describe the function of electricity and the agarose gel in electrophoresis?
The electricity pulls the polar DNA strands through the gel, and shorter DNA strands move farther because they are less inhibited by the gel. The gel acts as drag to separate the different length DNA strands, so different DNA creates specific dye bands.
What is the purpose of using a buffer in agarose gel electrophoresis?
The purpose of using a buffer in agarose gel electrophoresis is to maintain a stable pH and provide ions that help conduct electricity, allowing the DNA or other molecules to move through the gel.
What gel is typically used in electrophoresis experiments?
The gel typically used in electrophoresis experiments is agarose gel.
Process that restriction fragments of DNA are separated from each other by the use of electricity?
The process you are referring to is called electrophoresis. In this technique, DNA fragments are loaded onto a gel matrix and an electric current is applied. The negatively charged DNA molecules move towards the positive electrode, separating based on size and charge.
What was used before gel electrophoresis?
Before gel electrophoresis, techniques like paper electrophoresis and agarose slab gel electrophoresis were used for separating and analyzing DNA or proteins. These methods were less efficient and had lower resolution compared to gel electrophoresis.
Can a gel electrophoresis be performed both horizonnally and vertically?
yes for example 2D gel electrophoresis
What is used to sort DNA into different lenghts?
Gel Electrophoresis
Where can one learn more on gel electrophoresis?
To learn more about gel electrophoresis, one can Google it. There is also a whole Wikipedia article dedicated to gel electrophoresis, and it happens to be quite informative.
What causes the absence of bands in gel electrophoresis?
The absence of bands in gel electrophoresis can be caused by factors such as improper loading of samples, insufficient DNA concentration, or issues with the gel or electrophoresis equipment.
